PMID- 11452983
OWN - NLM
STAT- MEDLINE
DCOM- 20011217
LR  - 20191105
IS  - 0939-4451 (Print)
IS  - 0939-4451 (Linking)
VI  - 20
IP  - 4
DP  - 2001
TI  - Assignment of D-amino-acid oxidase gene to a human and a mouse chromosome.
PG  - 401-8
AB  - A part of D-amino-acid oxidase gene was amplified in the human and mouse by 
      polymerase chain reaction. The amplified fragments were ligated to plasmids and 
      then cloned. The plasmids containing the parts of D-amino-acid oxidase gene were 
      biotinylated and hybridized to human and mouse metaphase chromosomes. The 
      chromosomal slides were treated with fluorescein isothiocyanate (FITC)-conjugated 
      avidin. The hybridized signals were amplified with biotinylated anti-avidin 
      antibody and FITC-avidin. The chromosomes were counter-stained with 
      diamidino-phenylindole for assignment of the signal to a specific band. Using 
      this fluorescence in situ hybridization (FISH), D-amino-acid oxidase gene was 
      assigned to human chromosome 12q23-24.1 and mouse chromosome 5E3-F. Since these 
      regions are syntenic between human and mouse, the present results indicate that 
      the locus for this enzyme has been conserved through evolution.
FAU - Konno, R
AU  - Konno R
AD  - Department of Microbiology, Dokkyo University School of Medicine, Mibu, Tochigi, 
      Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Amino Acids
JT  - Amino acids
JID - 9200312
RN  - EC 1.4.3.- (DAO protein, human)
RN  - EC 1.4.3.- (Dao1 protein, mouse)
RN  - EC 1.4.3.3 (D-Amino-Acid Oxidase)
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 12
MH  - Cloning, Molecular
MH  - D-Amino-Acid Oxidase/*genetics/*metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Models, Genetic
MH  - Nucleic Acid Hybridization
MH  - Polymerase Chain Reaction
EDAT- 2001/07/17 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/07/17 10:00
PHST- 2001/07/17 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/07/17 10:00 [entrez]
AID - 10.1007/s007260170036 [doi]
PST - ppublish
SO  - Amino Acids. 2001;20(4):401-8. doi: 10.1007/s007260170036.