PMID- 11454426
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 128
IP  - 1
DP  - 2001 Jul 1
TI  - Amplification of hypoxia-inducible factor 1alpha gene in prostate cancer.
PG  - 31-4
AB  - Hypoxia-inducible factor 1 (HIF-1) is a transcription factor that regulates the 
      expression of genes associated with adaptation to reduced oxygen pressure. 
      Increased expression of HIF-1alpha gene (HIF1A) has been found in the majority of 
      prostate carcinomas. In addition, the PC-3 prostate cancer cell line has been 
      shown to express the gene even under normoxic conditions. By comparative genomic 
      hybridization (CGH), we have earlier shown that the PC-3 cell line contains a 
      high-level amplification in the chromosomal region harboring the HIF1A gene. 
      Here, we first fine mapped the gene to locus 14q23 by fluorescence in situ 
      hybridization (FISH). The gene was then shown to be highly amplified in the PC-3 
      cell line. Subsequently, the copy number of the HIF1A gene was studied in 5 other 
      prostate cancer cell lines (LNCaP, DU-145, NCI-H660, Tsu-Pr, JCA-1) and in 117 
      prostate tumors representing both hormone-dependent and -refractory disease as 
      well as primary and metastatic lesions. No high-level amplifications of the HIF1A 
      gene were found. Additional copies of the gene were seen in all of the cell lines 
      and in 36% of the tumors. There was no association between the tumor type and the 
      copy number alterations of the gene. In conclusion, high-level amplification of 
      the HIF1A gene may explain the overexpression of the gene in the PC-3 prostate 
      cancer cell line. However, such high-level amplification seems to be very rare in 
      prostate cancer.
FAU - Saramaki, O R
AU  - Saramaki OR
AD  - Laboratory of Cancer Genetics, Institute of Medical Technology, Hospital of the 
      University of Tampere, Tampere, Finland.
FAU - Savinainen, K J
AU  - Savinainen KJ
FAU - Nupponen, N N
AU  - Nupponen NN
FAU - Bratt, O
AU  - Bratt O
FAU - Visakorpi, T
AU  - Visakorpi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - *Chromosome Mapping
MH  - Chromosomes, Human, Pair 14/*genetics
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - Gene Amplification
MH  - Gene Dosage
MH  - Helix-Loop-Helix Motifs/*genetics
MH  - Humans
MH  - Hypoxia-Inducible Factor 1
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Nuclear Proteins/*genetics/metabolism
MH  - Nucleic Acid Hybridization
MH  - Prostatic Neoplasms/*genetics
MH  - Transcription Factors/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2001/07/17 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/07/17 10:00
PHST- 2001/07/17 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/07/17 10:00 [entrez]
AID - S016546080100396X [pii]
AID - 10.1016/s0165-4608(01)00396-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2001 Jul 1;128(1):31-4. doi: 
      10.1016/s0165-4608(01)00396-x.