PMID- 11455004
OWN - NLM
STAT- MEDLINE
DCOM- 20010830
LR  - 20061115
IS  - 0893-3952 (Print)
IS  - 0893-3952 (Linking)
VI  - 14
IP  - 7
DP  - 2001 Jul
TI  - Simultaneous paired analysis of numerical chromosomal aberrations and DNA content 
      in osteosarcoma.
PG  - 710-6
AB  - Relatively little is known about the biologic relevance of numerical chromosomal 
      changes in relation to DNA content in osteosarcoma. In this study, by using a 
      series of human osteosarcoma cell lines, we standardized a method for the 
      assessment, on the same nuclei specimen, of both specific chromosome copy numbers 
      by fluorescence in situ hybridization (FISH) and the DNA content by static 
      cytofluorometry or image cytometry. On the same cell lines, we also evaluated the 
      DNA content by using flow cytometry and the chromosome number distribution by 
      metaphase analysis. Comparison between these different methods showed that DNA 
      ploidy level as determined by FISH or metaphase analysis is frequently lower than 
      the ploidy pattern as defined by cytometric methods. By using comparative genomic 
      hybridization, we were able to demonstrate that these discrepancies were due to 
      the presence of several unbalanced chromosome aberrations, specifically gains and 
      high-level amplifications, which affect the total DNA content with less effect on 
      the total chromosome number. Thus, evaluation of DNA ploidy in osteosarcoma cells 
      is needed for a correct interpretation of FISH or cytogenetic data concerning 
      numerical chromosomal changes. Evaluation of tumor ploidy in a series of clinical 
      samples demonstrated that in high-grade osteosarcoma, flow cytometry sometimes 
      may give false results because of the presence of high proportions of 
      contaminating, nonneoplastic cells that cannot be excluded from the flow 
      cytometric assessment but that do not interfere with the evaluation of DNA ploidy 
      by static cytofluorometry or image cytometry, in which only tumor cells are 
      selected for the analysis. The possibility of using this method to evaluate, on 
      the same nuclei sample, both specific chromosomal aberrations and DNA ploidy may 
      allow a better determination of numerical chromosomal changes that may be 
      relevant for the biologic behavior of osteosarcoma.
FAU - Serra, M
AU  - Serra M
AD  - Laboratorio di Ricerca OncologicaIstituti Ortopedici Rizzoli, Bologna, Italy.
FAU - Tarkkanen, M
AU  - Tarkkanen M
FAU - Baldini, N
AU  - Baldini N
FAU - Scotlandi, K
AU  - Scotlandi K
FAU - Sarti, M
AU  - Sarti M
FAU - Maurici, D
AU  - Maurici D
FAU - Manara, M C
AU  - Manara MC
FAU - Benini, S
AU  - Benini S
FAU - Bacchini, P
AU  - Bacchini P
FAU - Knuutila, S
AU  - Knuutila S
FAU - Picci, P
AU  - Picci P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Bone Neoplasms/*genetics/pathology
MH  - Chromosome Aberrations/*genetics
MH  - DNA, Neoplasm/genetics/*metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Osteosarcoma/*genetics/pathology
MH  - Ploidies
MH  - Tumor Cells, Cultured
EDAT- 2001/07/17 10:00
MHDA- 2001/08/31 10:01
CRDT- 2001/07/17 10:00
PHST- 2001/07/17 10:00 [pubmed]
PHST- 2001/08/31 10:01 [medline]
PHST- 2001/07/17 10:00 [entrez]
AID - 10.1038/modpathol.3880377 [doi]
PST - ppublish
SO  - Mod Pathol. 2001 Jul;14(7):710-6. doi: 10.1038/modpathol.3880377.