PMID- 11455249
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20190713
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 71
IP  - 12
DP  - 2001 Jun 27
TI  - A novel strategy for organ allografts using sublethal (7 Gy) irradiation followed 
      by injection of donor bone marrow cells via portal vein.
PG  - 1725-31
AB  - A new strategy for organ allografts that does not require recourse to 
      immunosuppressants is established in mice. The strategy includes sublethal (7 Gy) 
      irradiation followed by the injection of donor bone marrow cells (BMCs) via the 
      portal vein (P.V.) and organ allografts 1 day after irradiation. Irradiation 
      doses (< or =7 Gy) are found to allow the recipients to survive without the need 
      to reconstitute the BMCs, as the recipient hematolymphoid cells can gradually 
      recover. One hundred percent of recipients irradiated with 7 Gy followed by 
      either P.V. or i.v. injection of donor BMCs accept organ allografts (the skin, 
      pancreas, and adrenal glands) for more than 1 year. However, organ allograft 
      survival rates decrease when irradiation doses are reduced; the skin graft 
      survival rate of mice treated with 6.5 Gy and P.V. injection of BMCs is 79%, 
      whereas that of mice treated with 6.5 Gy and i.v. injection is 50%, indicating 
      that the P.V. injection of BMCs induces persistent tolerance more effectively 
      than the i.v. injection. H-2 typing reveals that almost all the hematolymphoid 
      cells (>98%) in the peripheral blood and hematolymphoid organs are donor-derived 
      even 1 year after the treatment (7 Gy and P.V.). The T cells are tolerant to both 
      donor-type and host-type MHC determinants. The major mechanism underlying the 
      persistent tolerance induced by this strategy seems to be because of clonal 
      deletion. This simple and safe strategy would be of great advantage for human 
      organ transplantation.
FAU - Jin, T
AU  - Jin T
AD  - First Department of Pathology, Kansai Medical University, 10-15 Fumizono-cho, 
      Moriguchi City, Osaka 570-8506, Japan.
FAU - Toki, J
AU  - Toki J
FAU - Inaba, M
AU  - Inaba M
FAU - Sugiura, K
AU  - Sugiura K
FAU - Fan, T
AU  - Fan T
FAU - Yu, C
AU  - Yu C
FAU - Lian, Z
AU  - Lian Z
FAU - Takase, K
AU  - Takase K
FAU - Feng, B
AU  - Feng B
FAU - Ito, T
AU  - Ito T
FAU - Cui, Y
AU  - Cui Y
FAU - Yang, G
AU  - Yang G
FAU - Ikehara, S
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
SB  - IM
MH  - Adrenal Glands/transplantation
MH  - Animals
MH  - *Bone Marrow Transplantation/*methods
MH  - Chimera
MH  - Female
MH  - Graft Survival
MH  - Immune Tolerance
MH  - Injections, Intravenous
MH  - Mice
MH  - Mice, Inbred Strains
MH  - *Organ Transplantation
MH  - Pancreas Transplantation
MH  - *Portal Vein
MH  - *Preoperative Care
MH  - Skin Transplantation
MH  - Survival Analysis
MH  - Transplantation Immunology
MH  - Transplantation, Homologous
MH  - *Whole-Body Irradiation
EDAT- 2001/07/17 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/07/17 10:00
PHST- 2001/07/17 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/07/17 10:00 [entrez]
AID - 10.1097/00007890-200106270-00004 [doi]
PST - ppublish
SO  - Transplantation. 2001 Jun 27;71(12):1725-31. doi: 
      10.1097/00007890-200106270-00004.