PMID- 11455251
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20190713
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 71
IP  - 12
DP  - 2001 Jun 27
TI  - Differentiation of transplanted bone marrow cells in the adult mouse brain.
PG  - 1735-40
AB  - BACKGROUND: Bone marrow transplantation is reportedly effective in preventing the 
      progression of neurological deterioration in lysosomal storage disorders, 
      although the mechanism underlying the therapeutic effects remains to be 
      elucidated. Recent research on stem cell biology suggests that bone marrow cells 
      contain nonhematopoietic stem cells, including brain precursor cells. To evaluate 
      the contribution of bone marrow cells as carriers for cell and gene therapy of 
      neurological disorders, we studied the fate of transplanted bone marrow cells in 
      the adult mouse brain. METHODS: Bone marrow cells were genetically marked with a 
      retroviral vector containing the green fluorescence protein gene and then 
      transplanted into irradiated mice by either systemic infusion or direct 
      injection. To identify cell types, brain sections were stained with specific 
      antibodies against neuronal cell markers-neuron specific enolase for neurons, 
      glial fibrillary acidic protein (GFAP) for astrocytes, carbonic anhydrase II 
      (CAII) for oligodendrocytes, and ionized calcium binding adaptor molecule 1 
      (Iba1) for microglia-and then examined under a confocal microscope. RESULTS: 
      Twenty-four weeks after systemic infusion, transplanted cells expressed Iba1 but 
      none of the other brain cell markers. Conversely, 12 weeks after direct 
      injection, transplanted cells were stained with antibodies against GFAP, CAII, 
      and Iba1. CONCLUSIONS: Bone marrow contains cells capable of differentiating into 
      oligodendrocytes, astrocytes, and microglia when exposed to the brain 
      microenvironment. Autologous bone marrow cells may be useful as carriers for ex 
      vivo gene therapy for lysosomal disorders with neurological symptoms.
FAU - Nakano, K
AU  - Nakano K
AD  - Department of Biochemistry and Molecular Biology, Nippon Medical School, 1-1-5 
      Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan.
FAU - Migita, M
AU  - Migita M
FAU - Mochizuki, H
AU  - Mochizuki H
FAU - Shimada, T
AU  - Shimada T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Indicators and Reagents)
RN  - 0 (Luminescent Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*pathology
MH  - *Bone Marrow Transplantation
MH  - Brain/*surgery
MH  - Cell Differentiation
MH  - Corpus Striatum
MH  - Green Fluorescent Proteins
MH  - Indicators and Reagents
MH  - Injections
MH  - Injections, Intravenous
MH  - Luminescent Proteins/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neuroglia/pathology
MH  - Stereotaxic Techniques
MH  - Transduction, Genetic
EDAT- 2001/07/17 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/07/17 10:00
PHST- 2001/07/17 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/07/17 10:00 [entrez]
AID - 10.1097/00007890-200106270-00006 [doi]
PST - ppublish
SO  - Transplantation. 2001 Jun 27;71(12):1735-40. doi: 
      10.1097/00007890-200106270-00006.