PMID- 11456275
OWN - NLM
STAT- MEDLINE
DCOM- 20011207
LR  - 20190906
IS  - 0918-8959 (Print)
IS  - 0918-8959 (Linking)
VI  - 48
IP  - 2
DP  - 2001 Apr
TI  - TNF-alpha inhibits 3T3-L1 adipocyte differentiation without downregulating the 
      expression of C/EBPbeta and delta.
PG  - 249-53
AB  - Tumor necrosis factor-alpha (TNF-alpha) has been reported to inhibit adipocyte 
      differentiation in which multiple transcription factors including CCAAT enhancer 
      binding proteins (C/EBPs) and peroxisome proliferator-activated receptor (PPAR) 
      gamma play an important role. Induction of C/EBPalpha and PPARgamma, which 
      regulate the expression of many adipocyte-related genes, is dependent on the 
      expression of C/EBPbeta and C/EBPdelta at the early phase of adipocyte 
      differentiation. To elucidate the mechanism by which TNF-alpha inhibits adipocyte 
      differentiation, we examined the effect of TNF-alpha on the expression of these 
      transcription factors in mouse 3T3-L1 preadipocytes. TNF-alpha did not abrogate 
      the induction of C/EBPbeta and C/EBPdelta in response to differentiation stimuli. 
      In fully differentiated adipocytes, TNF-alpha rapidly induced C/EBPbeta and 
      C/EBPdelta, whereas it downregulated the expression of C/EBPalpha and PPARgamma. 
      Our results suggest that TNF-alpha inhibits adipocyte differentiation 
      independently of the downregulation of C/EBPbeta and C/EBPdelta.
FAU - Kurebayashi, S
AU  - Kurebayashi S
AD  - Department of Molecular Medicine, Osaka University Medical School, Suita, Japan.
FAU - Sumitani, S
AU  - Sumitani S
FAU - Kasayama, S
AU  - Kasayama S
FAU - Jetten, A M
AU  - Jetten AM
FAU - Hirose, T
AU  - Hirose T
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Endocr J
JT  - Endocrine journal
JID - 9313485
RN  - 0 (CCAAT-Enhancer-Binding Protein-beta)
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (Cebpd protein, mouse)
RN  - 0 (Nuclear Receptor Subfamily 1, Group F, Member 1)
RN  - 0 (Nuclear Receptor Subfamily 1, Group F, Member 3)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Receptors, Thyroid Hormone)
RN  - 0 (Rorc protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 1191-85-1 (5,8,11,14-Eicosatetraynoic Acid)
RN  - 142662-43-9 (CCAAT-Enhancer-Binding Protein-delta)
SB  - IM
MH  - 3T3 Cells
MH  - 5,8,11,14-Eicosatetraynoic Acid/pharmacology
MH  - Adipocytes/*cytology
MH  - Animals
MH  - CCAAT-Enhancer-Binding Protein-beta/*genetics
MH  - CCAAT-Enhancer-Binding Protein-delta
MH  - CCAAT-Enhancer-Binding Proteins/*genetics
MH  - *Cell Differentiation
MH  - Gene Expression Regulation/*drug effects
MH  - Mice
MH  - Nuclear Receptor Subfamily 1, Group F, Member 1
MH  - Nuclear Receptor Subfamily 1, Group F, Member 3
MH  - Receptors, Cytoplasmic and Nuclear/genetics
MH  - *Receptors, Retinoic Acid
MH  - *Receptors, Thyroid Hormone
MH  - Trans-Activators/genetics
MH  - Transcription Factors/genetics
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2001/07/18 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/07/18 10:00
PHST- 2001/07/18 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/07/18 10:00 [entrez]
AID - 10.1507/endocrj.48.249 [doi]
PST - ppublish
SO  - Endocr J. 2001 Apr;48(2):249-53. doi: 10.1507/endocrj.48.249.