PMID- 11459231
OWN - NLM
STAT- MEDLINE
DCOM- 20020221
LR  - 20240315
IS  - 1016-8478 (Print)
IS  - 1016-8478 (Linking)
VI  - 11
IP  - 3
DP  - 2001 Jun 30
TI  - Increase in the expression of human leukocyte antigen class I in human 
      fibroblasts by soluble factors secreted from human cytomegalovirus-infected 
      cells.
PG  - 392-8
AB  - The human cytomegalovirus (HCMV) is known to downregulate the expression of the 
      human leukocyte antigen (HLA) class I for escape from immune surveillance. In 
      order to understand the HCMV immune evasion mechanism, expression of HLA class I 
      on the surface of HCMV-infected cells was investigated. A decrease in the HLA 
      class I expression was observed at higher MOI; whereas at a lower MOI a slight 
      increase in the HLA class I expression was observed. When HCMV-infected and 
      uninfected cells were separately prepared on coverslips and co-cultured, the 
      increased HLA class I expression was observed in uninfected cells. Treatment of 
      the uninfected cells with the culture supernatant from HCMV-infected cells 
      resulted in an increase in the HLA class I expression. A biochemical analysis of 
      the HCMV-infected cell culture supernatant revealed the presence of interferon 
      (IFN) beta interleukin (IL)-1beta, and IL-6. The HLA class I-enhancing activity 
      of the culture supernatant was mimicked by IFN beta, but not by IL1-beta or IL-6, 
      and was partially reversed by pretreatment with an antibody to IFN beta. 
      Therefore, it appears that the HCMV infection of human foreskin fibroblast cells 
      induces interferon beta and other soluble factor(s) that are responsible for the 
      up-regulation of the HLA class I expression.
FAU - Lee, G C
AU  - Lee GC
AD  - Division of Life Sciences, Chungbuk National University, Cheongju, Korea.
FAU - Song, B H
AU  - Song BH
FAU - Lee, C H
AU  - Lee CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cells
JT  - Molecules and cells
JID - 9610936
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Cells, Cultured
MH  - Culture Media, Conditioned/pharmacology
MH  - Cytomegalovirus Infections/*immunology/metabolism
MH  - Down-Regulation/immunology
MH  - Fibroblasts/cytology/metabolism/virology
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique, Indirect
MH  - Histocompatibility Antigens Class I/*metabolism
MH  - Humans
MH  - Interferon-beta/*metabolism
MH  - Interleukin-1/metabolism
MH  - Interleukin-6/metabolism
MH  - Skin/cytology
MH  - Solubility
EDAT- 2001/07/19 10:00
MHDA- 2002/02/22 10:01
CRDT- 2001/07/19 10:00
PHST- 2001/07/19 10:00 [pubmed]
PHST- 2002/02/22 10:01 [medline]
PHST- 2001/07/19 10:00 [entrez]
AID - S1016-8478(23)17052-X [pii]
PST - ppublish
SO  - Mol Cells. 2001 Jun 30;11(3):392-8.