PMID- 11463453
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 128
IP  - 2
DP  - 2001 Jul 15
TI  - 6p abnormalities and TNF-alpha over-expression in retinoblastoma cell line.
PG  - 141-7
AB  - Retinoblastoma is the most common primary intra-ocular tumor in childhood. It has 
      been established that recurrent cytogenetic abnormalities are the hallmark of 
      mostly all malignant tumors. Recurrent atypical cytogenetic abnormalities of the 
      short arm of chromosome 6 were reported in several cases of retinoblastoma, 
      mainly in association with reciprocal translocations. In the present study, 
      alterations of chromosome 6 associated with retinoblastoma were studies in the 
      Y-79 and the WERI-Rb-1 cell lines established from highly malignant 
      retinoblastomas. Fluorescence in situ hybridization (FISH) technique was used to 
      identify the chromosomal breakpoint on 6p in this cell line. To perform this 
      detection, yeast artificial chromosome (YAC) clones from p21 to p22 bands were 
      used and the breakpoint was localized on 6p21.3. Previous studies had suggested 
      that activation of some genes on 6p could be dependent on a translocation 
      mechanism. Expression of a candidate gene localized near the chromosomal 
      breakpoint was measured by immunocytochemistry and flow cytometry techniques. An 
      enhancement of the tumor necrosis factor-alpha (TNF-alpha) protein expression in 
      Y79 cells was detected by this approach. The relationship between TNF-alpha 
      over-expression and the malignancy of retinoblastoma is discussed.
FAU - Imbert, I
AU  - Imbert I
AD  - Institut de Genetique Humaine, CNRS-UPR 1142, 141 rue de la Cardonille, 
      Montpellier, France.
FAU - Coignet, L J
AU  - Coignet LJ
FAU - Pellestor, F
AU  - Pellestor F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Blotting, Southern
MH  - Chromosome Aberrations/*genetics
MH  - Chromosomes, Human, Pair 6/*genetics
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Retinal Neoplasms/*genetics/metabolism
MH  - Retinoblastoma/*genetics/metabolism
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/biosynthesis/*genetics
EDAT- 2001/07/21 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/07/21 10:00
PHST- 2001/07/21 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/07/21 10:00 [entrez]
AID - S0165-4608(01)00402-2 [pii]
AID - 10.1016/s0165-4608(01)00402-2 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2001 Jul 15;128(2):141-7. doi: 
      10.1016/s0165-4608(01)00402-2.