PMID- 11463458
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 128
IP  - 2
DP  - 2001 Jul 15
TI  - Chromosomal gains and losses are uncommon in hairy cell leukemia: a study based 
      on comparative genomic hybridization and interphase fluorescence in situ 
      hybridization.
PG  - 164-7
AB  - In contrast to other subtypes of lymphoproliferative malignancies, the genetic 
      mechanisms underlying the pathogenesis of hairy cell leukemia (HCL) are unknown. 
      We studied densely infiltrated splenic tissue of 14 cases of HCL for the presence 
      of chromosomal gains and losses by comparative genomic hybridization (CGH). 
      Chromosomal imbalances were detected in only four of the 14 cases. Chromosomal 
      gains involved the regions 5q13-q31 (two cases) and 1p32-p36.2 (one case). A loss 
      of the region 11q14-q22 was found in one additional patient. The imbalances 
      affecting the regions 5q and 11q were confirmed by interphase fluorescence in 
      situ hybridization (FISH) using PAC clone 144G9 (5q31) and YAC clones 755B11 
      (11q22.3-q23.1) and 801E11 (11q22.3-q23.1 spanning the ATM gene) and occurred in 
      61% to 75% of analyzed nuclei. The latter DNA probes and probes hybridizing to 
      chromosomal regions, which are frequently deleted in other subtypes of 
      non-Hodgkin lymphomas (NHL), namely 9p21/ P16(INK4A), 13q14/D13S25, and 17p13/P53 
      were subsequently applied to all 14 cases of HCL, but no additional abnormalities 
      were found. We conclude that overrepresentation of chromosome 5 represents a 
      recurrent aberration in HCL and that the commonly overrepresented region resides 
      in 5q13-q31. Chromosomal imbalances including deletions of the tumor suppressor 
      gene loci 9p21/P16(INK4A), 13q14/D13S25, and 17p13/P53 rarely occur in HCL in 
      contrast to some other subtypes of B-cell NHL. The pathogenetic role of 11q/ATM 
      alterations in HCL remains to be determined.
FAU - Dierlamm, J
AU  - Dierlamm J
AD  - Department of Oncology and Hematology, University Hospital, Hamburg, Germany. 
      judith_dierlamm@yahoo.de
FAU - Stefanova, M
AU  - Stefanova M
FAU - Wlodarska, I
AU  - Wlodarska I
FAU - Michaux, L
AU  - Michaux L
FAU - Hinz, K
AU  - Hinz K
FAU - Penas, E M
AU  - Penas EM
FAU - Maes, B
AU  - Maes B
FAU - Hagemeijer, A
AU  - Hagemeijer A
FAU - De Wolf-Peeters, C
AU  - De Wolf-Peeters C
FAU - Hossfeld, D K
AU  - Hossfeld DK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Chromosome Aberrations/*genetics
MH  - Chromosomes, Human, Pair 5
MH  - Gene Deletion
MH  - Genes, Tumor Suppressor
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Leukemia, Hairy Cell/*genetics
MH  - Nucleic Acid Hybridization
MH  - Trisomy
EDAT- 2001/07/21 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/07/21 10:00
PHST- 2001/07/21 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/07/21 10:00 [entrez]
AID - S0165-4608(01)00415-0 [pii]
AID - 10.1016/s0165-4608(01)00415-0 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2001 Jul 15;128(2):164-7. doi: 
      10.1016/s0165-4608(01)00415-0.