PMID- 11467291
OWN - NLM
STAT- MEDLINE
DCOM- 20010816
LR  - 20190707
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 76
IP  - 1
DP  - 2001 Jul
TI  - An improved fixation technique for fluorescence in situ hybridization for 
      preimplantation genetic diagnosis.
PG  - 186-8
AB  - OBJECTIVE: To improve existing preimplantation genetic diagnosis fixation 
      techniques. DESIGN: Prospective randomized in vitro study. SETTING: Academic 
      medical center. PATIENT(S): None. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): 
      The intensity and clarity of fluorescence in situ hybridization (FISH) signals 
      and the percentage of successfully fixed blastomeres. RESULT(S): The described 
      fixation technique resulted in 100% fixation and 100% adequate FISH signals. Two 
      conventional techniques resulted in 94% and 87% fixation, and in adequate FISH 
      signals in 81% and 87%, respectively. CONCLUSION(S): This newly developed 
      fixation technique simplifies the process of fixation of blastomeres for 
      preimplantation diagnosis while essentially eliminating the possibility of losing 
      a cell during fixation. It will hopefully allow more IVF programs to offer their 
      patients preimplantation genetic diagnosis using the FISH technique.
FAU - Dozortsev, D I
AU  - Dozortsev DI
AD  - Division of Reproductive Endocrinology and Infertility, Department of Obstetrics 
      and Gynecology, Hutzel Hospital, Wayne State University, Detroit, MI 48201, USA. 
      aa4421@wayne.edu
FAU - McGinnis, K T
AU  - McGinnis KT
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Blastomeres
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Preimplantation Diagnosis/*methods
MH  - Prospective Studies
MH  - Random Allocation
MH  - Tissue Fixation/*methods
EDAT- 2001/07/27 10:00
MHDA- 2001/08/17 10:01
CRDT- 2001/07/27 10:00
PHST- 2001/07/27 10:00 [pubmed]
PHST- 2001/08/17 10:01 [medline]
PHST- 2001/07/27 10:00 [entrez]
AID - S0015-0282(01)01836-2 [pii]
AID - 10.1016/s0015-0282(01)01836-2 [doi]
PST - ppublish
SO  - Fertil Steril. 2001 Jul;76(1):186-8. doi: 10.1016/s0015-0282(01)01836-2.