PMID- 11467478
OWN - NLM
STAT- MEDLINE
DCOM- 20011207
LR  - 20170214
IS  - 0300-9858 (Print)
IS  - 0300-9858 (Linking)
VI  - 38
IP  - 4
DP  - 2001 Jul
TI  - Colocalization of porcine reproductive and respiratory syndrome virus and porcine 
      circovirus 2 in porcine dermatitis and nephrology syndrome by double-labeling 
      technique.
PG  - 436-41
AB  - Simultaneous detection of porcine reproductive and respiratory syndrome virus 
      (PRRSV) and porcine circovirus 2 (PCV2) was achieved by a double-labeling 
      technique using a combination of immunohistochemistry and in situ hybridization 
      in five pigs with naturally occurring porcine dermatitis and nephropathy syndrome 
      (PDNS). Both PRRSV and PCV2 were isolated from a homogenate of pooled skin and 
      kidney from three pigs. PRRSV RNA was demonstrated by reverse transcription 
      polymerase chain reaction (PCR) in skin, kidney, lymph node, and tonsil 
      homogenates from all pigs. PCV2 DNA was demonstrated by PCR in kidney, lymph 
      node, tonsil, liver, and lung homogenates from all pigs. For double-labeling 
      studies, the tissue samples were processed sequentially, first by 
      immunohistochemistry and then by in situ hybridization. The most consistent and 
      intense staining for PRRSV and PCV2 was in the kidney, lymph node, and tonsil. 
      PRRSV antigen and PCV2 DNA were also detected in the skin. This morphologic study 
      is the first to confirm the presence of both PRRSV and PCV2 in the same tissues 
      in pigs with naturally occurring PDNS.
FAU - Choi, C
AU  - Choi C
AD  - Department of Veterinary Pathology, College of Veterinary Medicine and School of 
      Agricultural Biotechnology, Seoul National University, Suwon, Republic of Korea.
FAU - Chae, C
AU  - Chae C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Vet Pathol
JT  - Veterinary pathology
JID - 0312020
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/analysis
MH  - Circoviridae Infections/pathology/*veterinary/virology
MH  - Circovirus/genetics/*isolation & purification
MH  - DNA, Viral/chemistry
MH  - Dermatitis/pathology/*veterinary/virology
MH  - Immunohistochemistry/veterinary
MH  - In Situ Hybridization/veterinary
MH  - Kidney/pathology/virology
MH  - Kidney Diseases/pathology/*veterinary/virology
MH  - Lymph Nodes/pathology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/pathology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification
MH  - RNA, Viral/chemistry/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Skin/pathology/virology
MH  - Swine
EDAT- 2001/07/27 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/07/27 10:00
PHST- 2001/07/27 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/07/27 10:00 [entrez]
AID - 10.1354/vp.38-4-436 [doi]
PST - ppublish
SO  - Vet Pathol. 2001 Jul;38(4):436-41. doi: 10.1354/vp.38-4-436.