PMID- 11467557
OWN - NLM
STAT- MEDLINE
DCOM- 20010823
LR  - 20190717
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 73
IP  - 13
DP  - 2001 Jul 1
TI  - Increasing bioanalytical throughput using pcSFC-MS/MS: 10 minutes per 96-well 
      plate.
PG  - 3083-8
AB  - The utility of packed-column supercritical, subcritical, and enhanced fluidity 
      liquid chromatographies (pcSFC) for high-throughput applications has increased 
      during the past few years. In contrast to traditional reversed-phase liquid 
      chromatography, the addition of a volatile component to the mobile phase, such as 
      CO2, produces a lower mobile-phase viscosity. This allows the use of higher flow 
      rates which can translate into faster analysis times. In addition, the resulting 
      mobile phase is considerably more volatile than the aqueous-based mobile phases 
      that are typically used with LC-MS, allowing the entire effluent to be directed 
      into the MS interface. High-throughput bioanalytical quantitation using 
      pcSFC-MS/MS for pharmacokinetics applications is demonstrated in this report 
      using dextromethorphan as a model compound. Plasma samples were prepared by 
      automated liquid/liquid extraction in the 96-well format prior to pcSFC-MS/MS 
      analysis. Three days of validation data are provided along with study sample data 
      from a patient dosed with commercially available Vicks 44. Using pcSFC and MS/MS, 
      dextromethorphan was quantified in 96-well plates at a rate of approximately 10 
      min/plate with average intraday accuracy of 9% or better. Daily relative standard 
      deviations (RSDs) were less than 10% for the 2.21 and 14.8 ng/mL quality control 
      (QC) samples, while the RSDs were less than 15% at the 0.554 ng/mL QC level.
FAU - Hoke, S H 2nd
AU  - Hoke SH 2nd
AD  - Health Care Research Center, The Procter & Gamble Company, Mason, Ohio 45040, 
      USA. hoke.sh@pg.com
FAU - Tomlinson, J A 2nd
AU  - Tomlinson JA 2nd
FAU - Bolden, R D
AU  - Bolden RD
FAU - Morand, K L
AU  - Morand KL
FAU - Pinkston, J D
AU  - Pinkston JD
FAU - Wehmeyer, K R
AU  - Wehmeyer KR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Antitussive Agents)
RN  - 7355X3ROTS (Dextromethorphan)
SB  - IM
MH  - Antitussive Agents/*blood/pharmacokinetics
MH  - Chromatography, Liquid/*methods
MH  - Dextromethorphan/*blood/pharmacokinetics
MH  - Humans
MH  - Mass Spectrometry/*methods
MH  - Quality Control
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2001/07/27 10:00
MHDA- 2001/08/24 10:01
CRDT- 2001/07/27 10:00
PHST- 2001/07/27 10:00 [pubmed]
PHST- 2001/08/24 10:01 [medline]
PHST- 2001/07/27 10:00 [entrez]
AID - 10.1021/ac0014820 [doi]
PST - ppublish
SO  - Anal Chem. 2001 Jul 1;73(13):3083-8. doi: 10.1021/ac0014820.