PMID- 11470923
OWN - NLM
STAT- MEDLINE
DCOM- 20010830
LR  - 20181113
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 98
IP  - 16
DP  - 2001 Jul 31
TI  - Molecular determinants for CC-chemokine recognition by a poxvirus CC-chemokine 
      inhibitor.
PG  - 9008-13
AB  - Poxviruses express a family of secreted proteins that bind with high affinity to 
      chemokines and antagonize the interaction with their cognate G protein-coupled 
      receptors (GPCRs). These viral inhibitors are novel in structure and, unlike 
      cellular chemokine receptors, are able to specifically interact with most, if not 
      all, CC-chemokines. We therefore sought to define the structural features of 
      CC-chemokines that facilitate this broad-spectrum interaction. Here, we identify 
      the residues present on human monocyte chemoattractant protein-1 (MCP-1) that are 
      required for high-affinity interaction with the vaccinia virus 35-kDa 
      CC-chemokine binding protein (VV-35kDa). Not only do these residues correspond to 
      those required for interaction with the cognate receptor CCR2b but they are also 
      conserved among many CC-chemokines. Thus, the results provide a structural basis 
      for the ability of VV-35kDa to promiscuously recognize CC-chemokines and block 
      binding to their receptors.
FAU - Seet, B T
AU  - Seet BT
AD  - Viral Immunology & Pathogenesis Laboratories, John P. Robarts Research Institute, 
      1400 Western Road, Room 133, London, ON, Canada N6G 2V4.
FAU - Singh, R
AU  - Singh R
FAU - Paavola, C
AU  - Paavola C
FAU - Lau, E K
AU  - Lau EK
FAU - Handel, T M
AU  - Handel TM
FAU - McFadden, G
AU  - McFadden G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20010724
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (CCI protein, Cowpox virus)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Viral Proteins)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Chemokine CCL2/*antagonists & inhibitors/chemistry
MH  - Humans
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Poxviridae/*chemistry
MH  - Protein Conformation
MH  - Sequence Homology, Amino Acid
MH  - Surface Plasmon Resonance
MH  - Viral Proteins/*pharmacology
MH  - Virulence Factors
PMC - PMC55364
EDAT- 2001/07/27 10:00
MHDA- 2001/08/31 10:01
PMCR- 2002/01/31
CRDT- 2001/07/27 10:00
PHST- 2001/07/27 10:00 [pubmed]
PHST- 2001/08/31 10:01 [medline]
PHST- 2001/07/27 10:00 [entrez]
PHST- 2002/01/31 00:00 [pmc-release]
AID - 171069398 [pii]
AID - 0693 [pii]
AID - 10.1073/pnas.171069398 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9008-13. doi: 
      10.1073/pnas.171069398. Epub 2001 Jul 24.