PMID- 11472067
OWN - NLM
STAT- MEDLINE
DCOM- 20011004
LR  - 20091119
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 75
IP  - 1-3
DP  - 2001 Jul
TI  - The myosin light chain kinase gene is not duplicated in mouse: partial structure 
      and chromosomal localization of Mylk.
PG  - 49-56
AB  - The gene encoding myosin light chain kinase (MYLK) is duplicated on human 
      chromosome 3 (HSA3; 3p13;3q21) and on a chromosome with conserved synteny to HSA3 
      in most non-human primate species. In human, the functional copy resides on 3q21, 
      whereas the 3p13 site contains a pseudogene. To trace the origin of the 
      duplication, we characterized the mouse gene Mylk. A single sequence 
      corresponding to the functional Mylk was detected. We sequenced a 180-kb 
      bacterial artificial chromosome clone containing the 24 first exons of Mylk; the 
      complete mouse gene is expected to span >200 kb. Comparisons with the draft of 
      the human genome revealed that the sequence and structure of MYLK are conserved 
      in mammals. Fluorescence in situ hybridization (FISH) analysis indicated that the 
      mouse gene localizes to a single site on chromosome 16B4-B5, a region with 
      conserved synteny with HSA3q. Our study provides information on both the 
      structure and the evolution of MYLK in mammals and suggests that it was 
      duplicated after the divergence of rodents and primates.
FAU - Giorgi, D
AU  - Giorgi D
AD  - IGH, CNRS UPR 1142, rue de la Cardonille, 34396 Montpellier, Cedex 5, France.
FAU - Ferraz, C
AU  - Ferraz C
FAU - Mattei, M G
AU  - Mattei MG
FAU - Demaille, J
AU  - Demaille J
FAU - Rouquier, S
AU  - Rouquier S
LA  - eng
SI  - GENBANK/AF335470
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - EC 2.7.11.18 (Myosin-Light-Chain Kinase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cattle
MH  - Chickens
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 3
MH  - Contig Mapping
MH  - Exons
MH  - *Gene Duplication
MH  - Gene Library
MH  - Genome
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Introns
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Myosin-Light-Chain Kinase/*genetics
MH  - Pseudogenes
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
EDAT- 2001/07/27 10:00
MHDA- 2001/10/05 10:01
CRDT- 2001/07/27 10:00
PHST- 2001/07/27 10:00 [pubmed]
PHST- 2001/10/05 10:01 [medline]
PHST- 2001/07/27 10:00 [entrez]
AID - S0888-7543(01)96571-1 [pii]
AID - 10.1006/geno.2001.6571 [doi]
PST - ppublish
SO  - Genomics. 2001 Jul;75(1-3):49-56. doi: 10.1006/geno.2001.6571.