PMID- 11472436
OWN - NLM
STAT- MEDLINE
DCOM- 20010906
LR  - 20220409
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 125
IP  - 1
DP  - 2001 Jul
TI  - Chemokines in human periodontal disease tissues.
PG  - 134-41
AB  - An immunoperoxidase technique was used to examine IP-10 (interferon-gamma 
      inducible protein 10), RANTES (regulated on activation normal T cell expressed 
      and secreted), MCP-1 (monocyte chemoattractant protein-1), and MIP-1 alpha 
      (macrophage inflammatory protein-1 alpha) in gingival biopsies from 21 
      healthy/gingivitis and 26 periodontitis subjects. The samples were placed into 3 
      groups according to the size of infiltrate. MIP-1 alpha+ cells were more abundant 
      than the other chemokines with few MCP-1+ cells. The mean percent MIP-1 alpha+ 
      cells was higher than the percent MCP-1+ cells (P = 0.02) in group 2 
      (intermediate size infiltrates) lesions from periodontitis subjects, other 
      differences not being significant due to the large variations between tissue 
      samples. Analysis of positive cells in relation to CD4/CD8 ratios showed that 
      with an increased proportion of CD8+ cells, the mean percent MIP-1 alpha+ cells 
      was significantly higher in comparison with the mean percent RANTES+ and MCP-1+ 
      cells (P < 0.015). Endothelial cells were MCP-1+ although positive capillaries 
      were found on the periphery of infiltrates only. Keratinocyte expression of 
      chemokines was weak and while the numbers of healthy/gingivitis and periodontitis 
      tissue sections positive for IP-10, RANTES and MCP-1 reduced with increasing 
      inflammation, those positive for MIP-1 alpha remained constant for all groups. In 
      conclusion, fewer leucocytes expressed MCP-1 in gingival tissue sections, 
      however, the percent MIP-1 alpha+ cells was increased particularly in tissues 
      with increased proportions of CD8 cells and B cells with increasing inflammation 
      and also in tissues with higher numbers of macrophages with little inflammation. 
      Further studies are required to determine the significance of MIP-1 alpha in 
      periodontal disease.
FAU - Gemmell, E
AU  - Gemmell E
AD  - Immunopathology Laboratory, Oral Biology and Pathology, School of Dentistry, The 
      University of Queensland, Brisbane, Australia. e.gemmell@mailbox.uq.edu.au
FAU - Carter, C L
AU  - Carter CL
FAU - Seymour, G J
AU  - Seymour GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Macrophage Inflammatory Proteins)
SB  - IM
MH  - Chemokine CCL2/*biosynthesis
MH  - Chemokine CCL4
MH  - Chemokine CCL5/*biosynthesis
MH  - Chemokine CXCL10
MH  - Chemokines, CXC/*biosynthesis
MH  - Connective Tissue/immunology/pathology
MH  - Endothelium, Vascular/cytology/immunology
MH  - Gingiva/immunology/pathology
MH  - Humans
MH  - Keratinocytes/cytology/immunology
MH  - Macrophage Inflammatory Proteins/*biosynthesis
MH  - Periodontal Diseases/*immunology/pathology
PMC - PMC1906105
EDAT- 2001/07/27 10:00
MHDA- 2001/09/08 10:01
PMCR- 2002/07/01
CRDT- 2001/07/27 10:00
PHST- 2001/07/27 10:00 [pubmed]
PHST- 2001/09/08 10:01 [medline]
PHST- 2001/07/27 10:00 [entrez]
PHST- 2002/07/01 00:00 [pmc-release]
AID - cei1511 [pii]
AID - 10.1046/j.1365-2249.2001.01511.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2001 Jul;125(1):134-41. doi: 10.1046/j.1365-2249.2001.01511.x.