PMID- 11472502
OWN - NLM
STAT- MEDLINE
DCOM- 20011018
LR  - 20191210
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 3
IP  - 6
DP  - 2001 Jun
TI  - Development of specific oligonucleotide probes for the identification and in situ 
      detection of hydrocarbon-degrading Alcanivorax strains.
PG  - 371-9
AB  - The genus Alcanivorax comprises diverse hydrocarbon-degrading marine bacteria. 
      Novel 16S rRNA-targeted oligonucleotide DNA probes (ALV735 and ALV735-b) were 
      developed to quantify two subgroups of the Alcanivorax/Fundibacter group by 
      fluorescence in situ hybridization (FISH), and the conditions for the 
      single-mismatch discrimination of the probes were optimized. The specificity of 
      the probes was improved further using a singly mismatched oligonucleotide as a 
      competitor. The growth of Alcanivorax cells in crude oil-contaminated sea water 
      under the biostimulation condition was investigated by FISH with the probe 
      ALV735, which targeted the main cluster of the Alcanivorax/Fundibacter group. The 
      size of the Alcanivorax population increased with increasing incubation time and 
      accounted for 91% of the 4',6-diamidino-2-phenylindole (DAPI) count after 
      incubation for 2 weeks. The probes developed in this study are useful for 
      detecting Alcanivorax populations in petroleum hydrocarbon-degrading microbial 
      consortia.
FAU - Syutsubo, K
AU  - Syutsubo K
AD  - Marine Biotechnology Institute, Kamaishi Laboratories, 3-75-1 Heita, Kamaishi, 
      Iwate 026-0001, Japan. stubo@kamaishi.mbio.co.jp
FAU - Kishira, H
AU  - Kishira H
FAU - Harayama, S
AU  - Harayama S
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Hydrocarbons)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Petroleum)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Base Sequence
MH  - DNA, Ribosomal/analysis
MH  - Gammaproteobacteria/*classification/*genetics/metabolism
MH  - Hydrocarbons/*metabolism
MH  - *In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes
MH  - Petroleum/*metabolism
MH  - RNA, Ribosomal, 16S/genetics
MH  - Seawater/microbiology
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Water Pollution, Chemical
EDAT- 2001/07/27 10:00
MHDA- 2001/10/19 10:01
CRDT- 2001/07/27 10:00
PHST- 2001/07/27 10:00 [pubmed]
PHST- 2001/10/19 10:01 [medline]
PHST- 2001/07/27 10:00 [entrez]
AID - emi204 [pii]
AID - 10.1046/j.1462-2920.2001.00204.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2001 Jun;3(6):371-9. doi: 10.1046/j.1462-2920.2001.00204.x.