PMID- 11472582
OWN - NLM
STAT- MEDLINE
DCOM- 20011004
LR  - 20191210
IS  - 1328-8067 (Print)
IS  - 1328-8067 (Linking)
VI  - 43
IP  - 4
DP  - 2001 Aug
TI  - Quantitation of human herpesvirus 6 DNA in infant with exanthem subitum by 
      microplate PCR-hybridization assay.
PG  - 372-8
AB  - BACKGROUND: Quantitative analysis of human herpesvirus 6 (HHV-6) genome is 
      important for monitoring active virus infection. The purpose of our study is to 
      evaluate the reliability of a hybridization-based microtiter plate assay 
      (polymerase chain reaction enzyme-linked immunosorbent assay (PCR ELISA)) for 
      quantifying the virus genome. METHODS: Semiquantitative analysis of the virus 
      genome was carried out in 31 (18 male and 13 female) infants with primary HHV-6 
      infection. If the HHV-6 virus could be isolated from the peripheral blood 
      mononuclear cells (PBMC), the infants were defined as being infected with HHV-6. 
      The PCR ELISA method was used to determine the virus load. A titration of the 
      virus was also carried out in the samples obtained during the acute phase of 
      exanthem subitum. RESULTS: Specificity of the method was demonstrated by a lack 
      of amplification of human herpesvirus 7 and cytomegalovirus DNA. The upper and 
      lower detection limits of the method were 58 and 5800 copies of the virus genome, 
      respectively. The quantity of HHV-6 DNA in the PBMC during the acute phase (879 
      +/- 975 copies/10(4) PBMC) was significantly higher than during the convalescent 
      phase (54 +/- 76 copies/10(4) PBMC). Furthermore, the virus load in acute phase 
      plasma (53 +/- 75 copies/microL) was also significantly higher than in the 
      convalescent phase samples (2 +/- 9 copies/microL). Virus load in both PBMC and 
      plasma gradually increased after the onset of exanthem subitum until about day 3 
      to 4 of the illness, but then decreased quickly. However, there was no 
      significant association between virus load and the numbers of infected cells. 
      CONCLUSION: Virus load in both PBMC and plasma gradually increased after the 
      onset of exanthem subitum until about day 3 and day 4 of the illness, 
      respectively, then it decreased quickly. These results indicate that our PCR 
      ELISA system is reliable for monitoring active HHV-6 infection in vivo.
FAU - Abe, T
AU  - Abe T
AD  - Department of Pediatrics, Fujita Health University Hospital, Toyoake, Aichi, 
      Japan.
FAU - Yoshikawa, T
AU  - Yoshikawa T
FAU - Ihira, M
AU  - Ihira M
FAU - Suzuki, K
AU  - Suzuki K
FAU - Suga, S
AU  - Suga S
FAU - Nishida, M
AU  - Nishida M
FAU - Nagata, M
AU  - Nagata M
FAU - Asano, Y
AU  - Asano Y
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Pediatr Int
JT  - Pediatrics international : official journal of the Japan Pediatric Society
JID - 100886002
RN  - 0 (DNA, Viral)
SB  - IM
MH  - DNA, Viral/*isolation & purification
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Exanthema Subitum/*genetics
MH  - Female
MH  - Genome, Viral
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Infant
MH  - Male
MH  - Polymerase Chain Reaction/*methods
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Viral Load
EDAT- 2001/07/27 10:00
MHDA- 2001/10/05 10:01
CRDT- 2001/07/27 10:00
PHST- 2001/07/27 10:00 [pubmed]
PHST- 2001/10/05 10:01 [medline]
PHST- 2001/07/27 10:00 [entrez]
AID - ped1401 [pii]
AID - 10.1046/j.1442-200x.2001.01401.x [doi]
PST - ppublish
SO  - Pediatr Int. 2001 Aug;43(4):372-8. doi: 10.1046/j.1442-200x.2001.01401.x.