PMID- 11473132
OWN - NLM
STAT- MEDLINE
DCOM- 20010830
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 21
IP  - 16
DP  - 2001 Aug 15
TI  - Synapse formation between central neurons requires postsynaptic expression of the 
      MEN1 tumor suppressor gene.
PG  - RC161
AB  - Synapse formation is a crucial step in the development of neuronal circuits and 
      requires precise coordination of presynaptic and postsynaptic activities. 
      However, molecular mechanisms that control the formation of functionally mature 
      synaptic contacts, in particular between central neurons, remain poorly 
      understood. To identify genes that are involved in the formation of central 
      synapses, we made use of molluscan neurons that in culture form synaptic contacts 
      between their somata (soma-soma synapses) in the absence of neurite outgrowth. 
      Using single-cell mRNA differential display, we have identified a molluscan 
      homolog of the multiple endocrine neoplasia type 1 (MEN1) tumor suppressor gene 
      encoding the transcription factor menin as a gene that is upregulated during 
      synapse formation. In vitro antisense knock-down of MEN1 mRNA blocks the 
      formation of mature synapses between different types of identified central 
      neurons. Moreover, immunocytochemistry and cell-specific knock-down of MEN1 mRNA 
      show that postsynaptic but not presynaptic expression is required for synapses to 
      form. Together, our data demonstrate that menin is a synaptogenic factor that is 
      critically involved in a general postsynaptic mechanism of synapse formation 
      between central neurons.
FAU - van Kesteren, R E
AU  - van Kesteren RE
AD  - Department of Molecular and Cellular Neurobiology, Research Institute 
      Neurosciences, Vrije Universiteit, 1081HV Amsterdam, The Netherlands. 
      revankes@bio.vu.nl
FAU - Syed, N I
AU  - Syed NI
FAU - Munno, D W
AU  - Munno DW
FAU - Bouwman, J
AU  - Bouwman J
FAU - Feng, Z P
AU  - Feng ZP
FAU - Geraerts, W P
AU  - Geraerts WP
FAU - Smit, A B
AU  - Smit AB
LA  - eng
SI  - GENBANK/AF395538
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Central Nervous System/cytology/*metabolism
MH  - Cloning, Molecular
MH  - Electrophysiology
MH  - Gene Expression Profiling
MH  - Genes, Tumor Suppressor
MH  - Immunohistochemistry
MH  - Lymnaea
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/*biosynthesis/genetics
MH  - Neurons/drug effects/*metabolism
MH  - Oligonucleotides, Antisense/pharmacology
MH  - *Proto-Oncogene Proteins
MH  - RNA, Messenger/antagonists & inhibitors/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Synapses/drug effects/*metabolism
MH  - Transcription Factors/biosynthesis/genetics
MH  - Up-Regulation/drug effects
PMC - PMC6763132
EDAT- 2001/07/27 10:00
MHDA- 2001/08/31 10:01
PMCR- 2002/02/15
CRDT- 2001/07/27 10:00
PHST- 2001/07/27 10:00 [pubmed]
PHST- 2001/08/31 10:01 [medline]
PHST- 2001/07/27 10:00 [entrez]
PHST- 2002/02/15 00:00 [pmc-release]
AID - 20015549 [pii]
AID - 5549 [pii]
AID - 10.1523/JNEUROSCI.21-16-j0004.2001 [doi]
PST - ppublish
SO  - J Neurosci. 2001 Aug 15;21(16):RC161. doi: 10.1523/JNEUROSCI.21-16-j0004.2001.