PMID- 11473996
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20240314
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 39
IP  - 8
DP  - 2001 Aug
TI  - Antibody-secreting cell responses to rotavirus proteins in gnotobiotic pigs 
      inoculated with attenuated or virulent human rotavirus.
PG  - 2807-13
AB  - Because of their similarities to infants in mucosal immune responses and their 
      susceptibility to human rotavirus (HRV) diarrhea, gnotobiotic pigs provide a 
      useful model for rotaviral disease. In this study, we performed quantitative 
      enzyme-linked immunospot (ELISPOT) assays to measure local and systemic 
      isotype-specific antibody-secreting cell (ASC) responses to individual structural 
      (VP4, VP6, and VP7) and nonstructural (NSP3 and NSP4) proteins of Wa HRV. The 
      Spodoptera frugiperda cells expressing each recombinant baculovirus HRV protein 
      were formalin fixed and used as antigen for ELISPOT assays. Neonatal gnotobiotic 
      pigs were orally inoculated once with virulent Wa (WaV) or three times with 
      attenuated Wa (WaA) HRV or mock inoculated (Mock) and then were challenged with 
      virulent Wa (WaV/PC) 28 days after the first inoculation. The ASCs from 
      intestinal and systemic lymphoid tissues of pigs from each group were quantitated 
      by ELISPOT assay at the day of challenge, at postinoculation day 28 (WaV, WaA, 
      and Mock) or at postchallenge day (PCD) 7 (WaV+WaV/PC, WaA+WaV/PC, and 
      Mock+WaV/PC). In all virus-inoculated pigs, regardless of the inoculum, lymphoid 
      tissue, or isotype, VP6 induced the highest numbers of ASCs, followed by VP4; 
      ASCs specific for VP7, NSP3, and NSP4 were less numerous. At challenge, total 
      HRV- and HRV protein-specific immunoglobulin A (IgA) and IgG ASCs in intestinal 
      lymphoid tissues were significantly greater in WaV- than in WaA-inoculated pigs, 
      and WaV pigs were fully protected against diarrhea postchallenge, whereas the WaA 
      pigs were partially protected. At PCD 7, there were no significant differences in 
      ASC numbers for any HRV proteins between the WaV+WaV/PC and WaA+WaV/PC groups.
FAU - Chang, K O
AU  - Chang KO
AD  - Food Animal Health Research Program, Ohio Agricultural Research and Development 
      Center/The Ohio State University, Wooster, Ohio 44691, USA.
FAU - Vandal, O H
AU  - Vandal OH
FAU - Yuan, L
AU  - Yuan L
FAU - Hodgins, D C
AU  - Hodgins DC
FAU - Saif, L J
AU  - Saif LJ
LA  - eng
GR  - R01 AI033561/AI/NIAID NIH HHS/United States
GR  - R01 AI037111/AI/NIAID NIH HHS/United States
GR  - R01AI33561/AI/NIAID NIH HHS/United States
GR  - R01AI37111/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antibody-Producing Cells/*immunology
MH  - Disease Models, Animal
MH  - *Germ-Free Life
MH  - Humans
MH  - Hybridomas
MH  - Rotavirus/*immunology/pathogenicity
MH  - Rotavirus Infections/*immunology/virology
MH  - *Swine
MH  - Viral Proteins/*immunology
MH  - Virus Shedding
PMC - PMC88243
EDAT- 2001/07/28 10:00
MHDA- 2002/01/05 10:01
PMCR- 2001/08/01
CRDT- 2001/07/28 10:00
PHST- 2001/07/28 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/07/28 10:00 [entrez]
PHST- 2001/08/01 00:00 [pmc-release]
AID - 0018 [pii]
AID - 10.1128/JCM.39.8.2807-2813.2001 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2001 Aug;39(8):2807-13. doi: 10.1128/JCM.39.8.2807-2813.2001.