PMID- 11474215
OWN - NLM
STAT- MEDLINE
DCOM- 20011011
LR  - 20181130
IS  - 0028-3835 (Print)
IS  - 0028-3835 (Linking)
VI  - 74
IP  - 2
DP  - 2001 Aug
TI  - Nitric oxide mediates the inhibitory effect of tumor necrosis factor-alpha on 
      prolactin release.
PG  - 82-6
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a pleiotropic cytokine that markedly 
      affects neuroendocrine functions. This cytokine is expressed in the anterior 
      pituitary where its receptors are also present. Nitric oxide (NO) is synthesized 
      in gonadotropes and folliculo-stellate cells of the anterior pituitary. Since NO 
      directly inhibits prolactin secretion, we investigated the involvement of NO in 
      the inhibitory effect of TNF-alpha on prolactin release from anterior pituitary 
      cells of female rats. The presence of L-NAME (1 mM), an inhibitor of NO synthase 
      (NOS), in the incubation medium significantly blunted the inhibition of prolactin 
      release produced by TNF-alpha (50 ng/ml). TNF-alpha increased nitrite release to 
      the incubation medium. The activity of NOS as measured by [(14)C]citrulline 
      production was significantly enhanced when anterior pituitary cells were 
      incubated with TNF-alpha for 8 h or more. Also, TNF-alpha induced iNOS gene 
      expression in anterior pituitary cells as assessed by reverse 
      transcriptase-polymerase chain reaction. The current results indicate that NO is 
      involved in the inhibitory effect of TNF-alpha on prolactin secretion and that 
      TNF-alpha induces iNOS transcription and stimulates NO synthesis in anterior 
      pituitary cells.
CI  - Copyright 2001 S. Karger AG, Basel
FAU - Theas, S
AU  - Theas S
AD  - Centro de Investigaciones en Reproduccion, Instituto de Biologia Celular y 
      Neurociencias, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, 
      Argentina.
FAU - De Laurentiis, A
AU  - De Laurentiis A
FAU - Candolfi, M
AU  - Candolfi M
FAU - Lopez, S L
AU  - Lopez SL
FAU - Carrasco, A E
AU  - Carrasco AE
FAU - Zaldivar, V
AU  - Zaldivar V
FAU - Seilicovich, A
AU  - Seilicovich A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Neuroendocrinology
JT  - Neuroendocrinology
JID - 0035665
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 9002-62-4 (Prolactin)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, rat)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Enzyme Inhibitors/metabolism/pharmacology
MH  - Female
MH  - Gene Expression/*drug effects/genetics
MH  - Nitric Oxide/agonists/*metabolism
MH  - Nitric Oxide Synthase/antagonists & inhibitors/genetics/*metabolism
MH  - Nitric Oxide Synthase Type II
MH  - Pituitary Gland/cytology
MH  - Prolactin/antagonists & inhibitors/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/drug effects/*metabolism/pharmacology
EDAT- 2001/07/28 10:00
MHDA- 2001/10/12 10:01
CRDT- 2001/07/28 10:00
PHST- 2001/07/28 10:00 [pubmed]
PHST- 2001/10/12 10:01 [medline]
PHST- 2001/07/28 10:00 [entrez]
AID - 54673 [pii]
AID - 10.1159/000054673 [doi]
PST - ppublish
SO  - Neuroendocrinology. 2001 Aug;74(2):82-6. doi: 10.1159/000054673.