PMID- 11476909
OWN - NLM
STAT- MEDLINE
DCOM- 20011011
LR  - 20190906
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 62
IP  - 8
DP  - 2001 Aug
TI  - Allele- and temperature-dependency of in vitro HLA class I assembly.
PG  - 858-68
AB  - Allelic variations of in vitro HLA class I assembly have been investigated in 
      both the absence and the presence of binding peptides by flow cytometry using 
      human leukocyte antigen (HLA) class I alpha chains isolated by alkali treatment 
      from cultured HLA homozygous B cells and polystyrene beads coated with anti-HLA 
      class I alpha chain antibodies specific to the C-terminal segment (anti-HLA class 
      I beads). The specificity of assembly was temperature dependent, while the 
      stability of the assembled complex depended on the bound peptide. The efficiency 
      of assembly was allele dependent and primarily ruled by the binding affinity of 
      alpha chains with beta(2)m. Thus, an allele hierarchy could be defined for the 
      binding of HLA-B alpha chain with beta(2)-microglobulin: B7, B18 > B35, B62 > 
      B27, B51. Allele and temperature dependency was found in HLA class I reassembly 
      on acid treated B cells. The HLA class I proteins, reassembled with specific 
      single peptides, could be efficiently transferred to anti-HLA class I beads. 
      These findings would be used to produce microspheres coupled at high surface 
      density with oriented single-peptide loaded HLA class I molecules and also to 
      improve the preparation efficiency of HLA class I tetramers by the use of 
      site-specific biotinylation.
FAU - Chersi, A
AU  - Chersi A
AD  - Regina Elena Institute for Cancer Research, Rome, Italy.
FAU - Garzillo, C
AU  - Garzillo C
FAU - Butler, R H
AU  - Butler RH
FAU - Tanigaki, N
AU  - Tanigaki N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA-A Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Peptides)
SB  - IM
MH  - *Alleles
MH  - B-Lymphocytes/immunology
MH  - Cells, Cultured
MH  - HLA-A Antigens/immunology
MH  - Histocompatibility Antigens Class I/*biosynthesis/genetics/immunology
MH  - Humans
MH  - In Vitro Techniques
MH  - Microspheres
MH  - Peptides/chemical synthesis/immunology/*metabolism
MH  - Protein Binding
MH  - Temperature
EDAT- 2001/07/31 10:00
MHDA- 2001/10/12 10:01
CRDT- 2001/07/31 10:00
PHST- 2001/07/31 10:00 [pubmed]
PHST- 2001/10/12 10:01 [medline]
PHST- 2001/07/31 10:00 [entrez]
AID - S0198-8859(01)00273-7 [pii]
AID - 10.1016/s0198-8859(01)00273-7 [doi]
PST - ppublish
SO  - Hum Immunol. 2001 Aug;62(8):858-68. doi: 10.1016/s0198-8859(01)00273-7.