PMID- 11477117
OWN - NLM
STAT- MEDLINE
DCOM- 20010830
LR  - 20190501
IS  - 0021-9746 (Print)
IS  - 1472-4146 (Electronic)
IS  - 0021-9746 (Linking)
VI  - 54
IP  - 8
DP  - 2001 Aug
TI  - Immunohistochemical assessment of human herpesvirus 8 infection in primary 
      central nervous system large B cell lymphomas.
PG  - 617-8
AB  - BACKGROUND/AIMS: Primary central nervous system large B cell lymphomas (PCNSLs) 
      are frequently associated with Epstein-Barr virus (EBV) in patients with AIDS and 
      less frequently in those without AIDS. Human herpesvirus 8 (HHV-8) has been 
      detected in these tumours by the polymerase chain reaction (PCR) at low copy 
      number, suggesting its presence in a cell compartment other than the malignant 
      one. The aim of this study was to use immunohistochemistry to assess HHV-8 
      infection in a series of PCNSLs from patients with and without AIDS. METHODS: The 
      antibody LN53, which reacts with the latent nuclear antigen 1 (LNA1) of HHV-8, 
      was used on tissue sections from 35 patients (17 with and 18 without AIDS) with 
      PCNSL. In addition, DNA was available for PCR (open reading frame 26 (ORF 26), 
      ORF 72, ORF 75) in three patients (two without AIDS, one with AIDS). RESULTS: 
      None of the 35 cases contained either DNA sequences or LNA1 positive cells. 
      CONCLUSIONS: These results confirm the lack of HHV-8 infection in tumour cells of 
      PCNSL. In addition, HHV-8 infected bystander cells do not express LNA1 latent 
      protein in this setting.
FAU - Gomez-Brouchet, A
AU  - Gomez-Brouchet A
AD  - Department of Pathology, CHU Rangueil, 31403 Toulouse, France. 
      BROUCHET.Anne@chui-toulouse.fr
FAU - Delisle, M B
AU  - Delisle MB
FAU - Vital, A
AU  - Vital A
FAU - Brousset, P
AU  - Brousset P
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Clin Pathol
JT  - Journal of clinical pathology
JID - 0376601
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (latent nuclear antigen (LNA))
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Brain Neoplasms/*virology
MH  - Herpesvirus 8, Human/*isolation & purification
MH  - Humans
MH  - Immunohistochemistry
MH  - Lymphoma, AIDS-Related/*virology
MH  - Lymphoma, B-Cell/*virology
MH  - Lymphoma, Large B-Cell, Diffuse/*virology
MH  - Nuclear Proteins/analysis/immunology
MH  - *Phosphoproteins
MH  - Rats
PMC - PMC1731488
EDAT- 2001/07/31 10:00
MHDA- 2001/08/31 10:01
PMCR- 2004/08/01
CRDT- 2001/07/31 10:00
PHST- 2001/07/31 10:00 [pubmed]
PHST- 2001/08/31 10:01 [medline]
PHST- 2001/07/31 10:00 [entrez]
PHST- 2004/08/01 00:00 [pmc-release]
AID - 10.1136/jcp.54.8.617 [doi]
PST - ppublish
SO  - J Clin Pathol. 2001 Aug;54(8):617-8. doi: 10.1136/jcp.54.8.617.