PMID- 11477658
OWN - NLM
STAT- MEDLINE
DCOM- 20011011
LR  - 20061115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 32
IP  - 1
DP  - 2001 Sep
TI  - Molecular cytogenetic definition of three distinct chromosome arm 14q deletion 
      intervals in gastrointestinal stromal tumors.
PG  - 26-32
AB  - Gastrointestinal stromal tumors (GISTs) are mesenchymal neoplasms characterized 
      by frequent chromosome arm 14q losses. In this study, the 14q changes in a series 
      of 39 histologically and immunohistochemically confirmed GISTs were analyzed in 
      detail by metaphase and/or interphase fluorescence in situ hybridization (FISH) 
      studies using 21 genetically well-characterized, region-specific 14q11-24 YAC 
      clones. By conventional cytogenetic analysis, acquired clonal chromosome 
      aberrations were found in 17 out of 35 tumors. Chromosome 14 was involved in 13 
      cases; six specimens showed complete chromosome 14 loss, while the remaining 
      seven had structural abnormalities with the breakpoints residing within the 
      intervals 14q11-13 or 14q22-24. Other recurrent chromosome aberrations included 
      frequent deletions of chromosome 1p (11/17), losses of chromosome 22 (7/17), 
      losses or deletions of chromosome arm 13 (6/17) or 15 (4/17), and gains or 
      translocations involving chromosome 17 (4/17). Combining cytogenetic data with 
      double-color FISH analysis, total or partial losses of 14q material were detected 
      in 29 out of 36 tumors (81%). The 14q losses were found in all stages and 
      histological subtypes. Two most frequent common deletion regions flanked by YACs 
      931B1 and 761D4, and 802E7 and 892C11 at 14q23-24 (25/30 of each; 83%) could be 
      identified. Furthermore, 21 tumors (70%) shared a region of deletion defined by 
      YACs 957H10 and 931E5 at 14q11-12. Our results suggest the presence of at least 
      three distinct critical deletion regions on chromosome 14 in GISTs.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Debiec-Rychter, M
AU  - Debiec-Rychter M
AD  - Center for Human Genetics, Katholieke Universiteit Leuven, Belgium. 
      Maria.Debiec-Rychter@med.kuleuven.ac.be
FAU - Sciot, R
AU  - Sciot R
FAU - Pauwels, P
AU  - Pauwels P
FAU - Schoenmakers, E
AU  - Schoenmakers E
FAU - Dal Cin, P
AU  - Dal Cin P
FAU - Hagemeijer, A
AU  - Hagemeijer A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosome Deletion
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 14/*genetics
MH  - Cytogenetic Analysis/*methods
MH  - Female
MH  - Gastrointestinal Neoplasms/*genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Middle Aged
MH  - Stromal Cells/pathology
EDAT- 2001/07/31 10:00
MHDA- 2001/10/12 10:01
CRDT- 2001/07/31 10:00
PHST- 2001/07/31 10:00 [pubmed]
PHST- 2001/10/12 10:01 [medline]
PHST- 2001/07/31 10:00 [entrez]
AID - 10.1002/gcc.1163 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2001 Sep;32(1):26-32. doi: 10.1002/gcc.1163.