PMID- 11478803
OWN - NLM
STAT- MEDLINE
DCOM- 20010913
LR  - 20240514
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 285
IP  - 5
DP  - 2001 Aug 3
TI  - The HGF/SF-induced phosphorylation of paxillin, matrix adhesion, and invasion of 
      prostate cancer cells were suppressed by NK4, an HGF/SF variant.
PG  - 1330-7
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) plays a crucial role in cancer 
      cell migration, matrix adhesion, invasion, and angiogenesis, via the 
      phosphorylation of the c-met tyrosine kinase. This study examined the ability of 
      NK4, a recently discovered HGF/SF variant, to inhibit the influence of HGF/SF on 
      cell-matrix interaction, paxillin phosphorylation, and invasion of prostate 
      cancer cells. HGF/SF was shown to dramatically enhance tumour cell motility, 
      invasion, cell-matrix adhesion, together with an increase in the degree of 
      paxillin phosphorylation and formation of focal adhesion complexes. However, 
      these HGF/SF-induced effects were suppressed by the presence of NK4. NK4 
      effectively inhibited the degree of HGF/SF-induced paxillin phosphorylation and 
      matrix adhesion. As a consequence, the matrix invasion of these prostate cancer 
      cells was also suppressed by NK4. In conclusion, this study shows that these 
      HGF/SF-enhanced events, which are critical steps in metastasis, can be inhibited 
      through the addition of NK4, thus warranting further in vivo studies on the 
      implication of NK4 as a potential antimetastasis agent in prostate cancer.
CI  - Copyright 2001 Academic Press.
FAU - Parr, C
AU  - Parr C
AD  - Department of Surgery, Department of Oncology, University of Wales College of 
      Medicine, Heath Park, Cardiff, CF14-4XN, United Kingdom. parrc@cardiff.ac.uk
FAU - Davies, G
AU  - Davies G
FAU - Nakamura, T
AU  - Nakamura T
FAU - Matsumoto, K
AU  - Matsumoto K
FAU - Mason, M D
AU  - Mason MD
FAU - Jiang, W G
AU  - Jiang WG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (HGF protein, human)
RN  - 0 (Mitogens)
RN  - 0 (PXN protein, human)
RN  - 0 (Paxillin)
RN  - 0 (Phosphoproteins)
RN  - 42HK56048U (Tyrosine)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - Blotting, Western
MH  - Cell Adhesion/drug effects
MH  - Cell Division/drug effects
MH  - Cell Movement/*drug effects
MH  - Cytoskeletal Proteins/analysis/*metabolism
MH  - Extracellular Matrix/*metabolism
MH  - Fluorescent Antibody Technique
MH  - Hepatocyte Growth Factor/antagonists & inhibitors/*pharmacology
MH  - Humans
MH  - Male
MH  - *Mitogens
MH  - Neoplasm Invasiveness
MH  - Paxillin
MH  - Phosphoproteins/analysis/*metabolism
MH  - Phosphorylation/drug effects
MH  - Prostatic Neoplasms/*drug therapy/*metabolism/pathology
MH  - Tumor Cells, Cultured
MH  - Tyrosine/metabolism
EDAT- 2001/08/02 10:00
MHDA- 2001/09/14 10:01
CRDT- 2001/08/02 10:00
PHST- 2001/08/02 10:00 [pubmed]
PHST- 2001/09/14 10:01 [medline]
PHST- 2001/08/02 10:00 [entrez]
AID - S0006-291X(01)95307-0 [pii]
AID - 10.1006/bbrc.2001.5307 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2001 Aug 3;285(5):1330-7. doi: 
      10.1006/bbrc.2001.5307.