PMID- 11480561
OWN - NLM
STAT- MEDLINE
DCOM- 20010816
LR  - 20190915
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 15
IP  - 8
DP  - 2001 Aug
TI  - Chromosomal instability in chromosome band 12p13: multiple breaks leading to 
      complex rearrangements including cytogenetically undetectable sub-clones.
PG  - 1193-202
AB  - During fluorescence in situ hybridization (FISH) analysis of metaphase cells from 
      70 patients with lymphoid and myeloid hematologic malignancies and chromosomal 
      rearrangements involving band 12p13, we identified nine patients (four with 
      lymphoid malignancies, four with myeloid malignancies and one with biphenotypic 
      leukemia) who showed more complicated rearrangements than we had expected from 
      conventional cytogenetic study. In six patients, multiple breaks occurred in 
      small segments of 12p with subsequent translocations and insertions of these 
      segments into other chromosomes, sometimes to unexpected regions. In three 
      patients additional chromosome breaks resulted in a sub-clone which was 
      cytogenetically indistinguishable from the main clone in each patient based on 
      the cytogenetic analysis. These subtle molecular events were detected exclusively 
      in a region covering TEL/ETV6 and KIP1/CDKN1B. Seven of nine had a previous 
      history of chemo/radiotherapy; all the patients showed complex karyotypes, even 
      though they were newly diagnosed with leukemia. Survival data were available in 
      five patients, and all survived less than 6 months. These findings suggest that 
      the 12p13 region, especially the above-mentioned region, is genetically unstable 
      and fragile. It is likely that multiple chromosome breaks were induced through 
      mutagens used in chemo/ radiotherapy, and are associated with a sub-group of 
      patients with an extremely bad prognosis.
FAU - Sato, Y
AU  - Sato Y
AD  - Department of Clinical Pathology, Research Institute of International Medical 
      Center of Japan, Tokyo.
FAU - Kobayashi, H
AU  - Kobayashi H
FAU - Suto, Y
AU  - Suto Y
FAU - Olney, H J
AU  - Olney HJ
FAU - Davis, E M
AU  - Davis EM
FAU - Super, H G
AU  - Super HG
FAU - Espinosa, R 3rd
AU  - Espinosa R 3rd
FAU - Le Beau, M M
AU  - Le Beau MM
FAU - Rowley, J D
AU  - Rowley JD
LA  - eng
GR  - CA40046/CA/NCI NIH HHS/United States
GR  - CA42557/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
SB  - IM
MH  - *Chromosome Fragility
MH  - *Chromosomes, Human, Pair 12
MH  - Gene Rearrangement
MH  - Hematologic Neoplasms/*genetics
MH  - Humans
EDAT- 2001/08/02 10:00
MHDA- 2001/08/17 10:01
CRDT- 2001/08/02 10:00
PHST- 2001/08/02 10:00 [pubmed]
PHST- 2001/08/17 10:01 [medline]
PHST- 2001/08/02 10:00 [entrez]
AID - 10.1038/sj.leu.2402188 [doi]
PST - ppublish
SO  - Leukemia. 2001 Aug;15(8):1193-202. doi: 10.1038/sj.leu.2402188.