PMID- 11481656
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20201208
IS  - 0260-437X (Print)
IS  - 0260-437X (Linking)
VI  - 21
IP  - 4
DP  - 2001 Jul-Aug
TI  - Allergenicity testing of supermethrin, phenoxyacetic acid and DNCB using in vivo 
      and in vitro modifications of the local lymph node assays, maximization and 
      epicutaneous testing.
PG  - 257-62
AB  - The purpose of this study was to compare two methods of testing for 
      allergenicity: in vivo and in vitro modifications of local lymph node assays 
      (LLNA) in mice and the maximization and epicutaneous skin tests in guinea pigs as 
      per the Organization for Economic Cooperation and Development (1981). Two 
      pesticides-the synthetic pyrethroid insecticide supermethrin (SM) and the 
      herbicide phenoxyacetic acid (PAA)-were evaluated using this testing battery. 
      1-Chloro-2,4-dinitrobenzene (DNCB) was selected as a reference allergen for the 
      local lymph node assay. In vitro modification of LLNA proliferative response per 
      standard cell count in lymphocyte cultures derived from treated Balb/c mice did 
      not differ from control mice. Results of the in vivo modification showed that 
      treatment with 50% PAA and 50% SM resulted in a lower proliferation response of 
      lymphocytes in lymph nodes compared with control animals. The vigour of the 
      proliferative response varied more in in vivo modification of LLNA. Stimulation 
      indices were <3, so PAA and SM did not indicate classification as allergens. 
      Lymphocyte proliferation in 1% DNCB-activated lymph nodes was approximately 
      fivefold higher than in those derived from control mice. Proliferation response 
      in vitro calculated as stimulation index was higher in DNCB-treated mice than 
      those observed in vivo, but differences were not dramatic. Auricular lymph node 
      weight and cellularity in mice treated with PAA and SM were similar to controls. 
      The DNCB stimulation index for lymph node cellularity was 5.5. Lymph node weight 
      was three times higher in comparison with controls. In the maximization test in 
      guinea pigs SM and PAA acid resulted in 40% and 50% of animals demonstrating 
      sensitization, respectively. Epicutaneous administration resulted in weaker 
      reaction. Both SM and PAA are mildly strong sensitizers by this battery.
CI  - Copyright 2001 John Wiley & Sons, Ltd.
FAU - Kuricova, M
AU  - Kuricova M
AD  - Institute of Preventive and Clinical Medicine, Department of Immunotoxicology, 
      Limbova 14, 83301 Bratislava, Slovak Republic. kuric@upkm.sk
FAU - Tulinska, J
AU  - Tulinska J
FAU - Kubova, J
AU  - Kubova J
FAU - Liskova, A
AU  - Liskova A
FAU - Fuortes, L
AU  - Fuortes L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Acetates)
RN  - 0 (Allergens)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (Insecticides)
RN  - 0 (Irritants)
RN  - 0 (Pyrethrins)
RN  - 1TR49121NP (cypermethrin)
RN  - YRC253429Q (phenoxyacetic acid)
SB  - IM
MH  - Acetates/administration & dosage/*pharmacology
MH  - Allergens/*pharmacology
MH  - Animals
MH  - Cell Division/drug effects
MH  - Dinitrochlorobenzene/administration & dosage/*pharmacology
MH  - Female
MH  - Guinea Pigs
MH  - In Vitro Techniques
MH  - Insecticides/*pharmacology
MH  - Irritants/pharmacology
MH  - Lymph Nodes/cytology/*drug effects/growth & development/immunology
MH  - Lymphocytes/drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Organ Size/drug effects
MH  - Pyrethrins/administration & dosage/*pharmacology
MH  - Reference Standards
MH  - Skin Tests/classification/*methods
EDAT- 2001/08/02 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/08/02 10:00
PHST- 2001/08/02 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/08/02 10:00 [entrez]
AID - 10.1002/jat.751 [pii]
AID - 10.1002/jat.751 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2001 Jul-Aug;21(4):257-62. doi: 10.1002/jat.751.