PMID- 11482613
OWN - NLM
STAT- MEDLINE
DCOM- 20011214
LR  - 20191210
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 13
IP  - 3
DP  - 2001 May
TI  - Comparison of porcine reproductive and respiratory syndrome virus growth in media 
      supplemented with fetal bovine serum or a serum replacement.
PG  - 276-9
AB  - Commercially available serum replacements are often used in cell culture as a 
      cheaper and less variable substitute for fetal bovine serum (FBS). The growth of 
      porcine reproductive and respiratory syndrome virus (PRRSV) isolates in CRL11171 
      cells maintained in a medium supplemented with FBS was compared with virus 
      propagation in the same cell line maintained in the same medium with a serum 
      replacement. The PRRSV replicated significantly better when the cell culture 
      medium was supplemented with FBS. The results of this study have implications for 
      the use of serum replacement-supplemented medium for PRRSV diagnosis by virus 
      isolation.
FAU - Schommer, S K
AU  - Schommer SK
AD  - Department of Veterinary Microbiology and Preventive Medicine, Veterinary Medical 
      Research Institute, College of Veterinary Medicine, Iowa State University, Ames 
      50011, USA.
FAU - Carpenter, S L
AU  - Carpenter SL
FAU - Paul, P S
AU  - Paul PS
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - 0 (Culture Media)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Culture Techniques/methods
MH  - *Culture Media
MH  - Fetal Blood
MH  - Porcine respiratory and reproductive syndrome virus/isolation & 
      purification/pathogenicity/*physiology
MH  - Specimen Handling
EDAT- 2001/08/03 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/08/03 10:00
PHST- 2001/08/03 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/08/03 10:00 [entrez]
AID - 10.1177/104063870101300320 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2001 May;13(3):276-9. doi: 10.1177/104063870101300320.