PMID- 11483253
OWN - NLM
STAT- MEDLINE
DCOM- 20011212
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 92
IP  - 1-2
DP  - 2001 Aug 15
TI  - Differential usage of multiple brain-derived neurotrophic factor promoter in rat 
      dorsal root ganglia following peripheral nerve injuries and inflammation.
PG  - 167-71
AB  - The brain-derived neurotrophic factor (BDNF) may act as either an autocrine or 
      paracrine survival factor for the dorsal root ganglion (DRG) neurons and may also 
      serve as a neurotransmitter or neuromodulator within the dorsal horn of the 
      spinal cord. The rat BDNF gene consists of four short 5' exons linked to separate 
      promoters and one 3' exon encoding the mature BDNF protein. An exon-specific 
      reverse transcription-polymerase chain reaction analysis was used to study the 
      differential utilization of multiple promoters in the DRG following unilateral 
      sciatic axotomy, dorsal rhizotomy and peripheral inflammation. The exon I 
      transcript showed the highest induction rate with the ipsilateral expression 
      elevated 4.3-5.8 times that of contralateral expression. Both exon II and III 
      mRNAs showed a smaller increase 1 day after the three kinds of stimuli. In 
      addition, exon IV mRNA transcription increased slightly only after rhizotomy, but 
      not after axotomy and peripheral inflammation after 1 day. Furthermore, the 
      elevated exon I mRNA levels 1 day after rhizotomy were sustained for up to 7 
      days. In contrast, those of the exon I mRNA after axotomy had declined 2.8 times 
      the control level after 7 days. These findings suggest that the promoter linked 
      to exon I may provide a major regulatory point of BDNF mRNA expression by 
      peripheral nerve injuries and inflammation. In addition, both exon I and IV mRNA 
      expression may show different temporal activation patterns according to the types 
      of injury.
FAU - Kim, D S
AU  - Kim DS
AD  - Department of Anatomy, School of Medicine, Kyungpook National University, 2-101 
      Dongin Dong, Taegu, 700-422, South Korea.
FAU - Lee, S J
AU  - Lee SJ
FAU - Cho, H J
AU  - Cho HJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 9007-81-2 (Freund's Adjuvant)
SB  - IM
MH  - Animals
MH  - Axotomy
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Exons/genetics
MH  - Freund's Adjuvant/toxicity
MH  - Ganglia, Spinal/*metabolism
MH  - *Gene Expression Regulation
MH  - Inflammation/chemically induced/genetics/*metabolism
MH  - Male
MH  - *Promoter Regions, Genetic
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Rhizotomy
MH  - Sciatic Nerve/*injuries
MH  - Spinal Nerve Roots/*injuries
MH  - *Transcription, Genetic
EDAT- 2001/08/03 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/08/03 10:00
PHST- 2001/08/03 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/08/03 10:00 [entrez]
AID - S0169328X01001541 [pii]
AID - 10.1016/s0169-328x(01)00154-1 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 2001 Aug 15;92(1-2):167-71. doi: 
      10.1016/s0169-328x(01)00154-1.