PMID- 11484160
OWN - NLM
STAT- MEDLINE
DCOM- 20090312
LR  - 20010802
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 18
IP  - 4
DP  - 2001 Aug
TI  - [Detection of monosomy 7 or 7q- in cases of myelodysplastic syndrome].
PG  - 255-8
AB  - OBJECTIVE: To investigate the value of interphase fluorescence in situ 
      hybridization(FISH) in the detection of monosomy 7 (-7)or deletion of long arm of 
      chromosome 7(7q-) in the cases of myelodysplastic syndrome (MDS). METHODS: 
      Forty-six cases of MDS and 10 normal controls were studied simultaneously by 
      conventional karyotype analysis and interphase FISH technique using SpectrumRed 
      directly labeled DNA specific probe for 7q32. Two hundred interphase cells were 
      analyzed for each case and the cells with one red hybridization spot<7% were 
      regarded as positive. RESULTS: Three cases displayed -7/7q- by conventional 
      cytogenetics(CC) and were confirmed by interphase FISH. Six cases in 43 cases who 
      did not show -7/7q- by CC displayed -7/7q- by interphase FISH. CONCLUSION: 
      Interphase FISH is very useful for the detection of -7 or 7q- in MDS and it is 
      more sensitive than CC.
FAU - Shen, Y
AU  - Shen Y
AD  - First Affiliated Hospital of Suzhou University, Jiangsu Institute of Hematology, 
      Suzhou, Jiangsu 215006 P.R.China. uujihsmc@public1.sz.js.cn
FAU - Xue, Y
AU  - Xue Y
FAU - Li, J
AU  - Li J
FAU - Guo, Y
AU  - Guo Y
FAU - Pan, J
AU  - Pan J
FAU - Wu, Y
AU  - Wu Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Chromosomes, Human, Pair 7/*genetics
MH  - Cytogenetic Analysis
MH  - DNA Probes
MH  - Female
MH  - Humans
MH  - In Situ Hybridization
MH  - *Karyotyping
MH  - Male
MH  - Middle Aged
MH  - *Monosomy
MH  - Myelodysplastic Syndromes/*genetics
MH  - Young Adult
EDAT- 2001/08/03 10:00
MHDA- 2009/03/13 09:00
CRDT- 2001/08/03 10:00
PHST- 2001/08/03 10:00 [pubmed]
PHST- 2009/03/13 09:00 [medline]
PHST- 2001/08/03 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2001 Aug;18(4):255-8.