PMID- 11484377
OWN - NLM
STAT- MEDLINE
DCOM- 20011004
LR  - 20170207
IS  - 1252-607X (Print)
IS  - 1252-607X (Linking)
VI  - 8
IP  - 2 Suppl
DP  - 2001 Jun
TI  - Estimating the genetic divergence and identification of three Trichinella species 
      by isoenzyme analysis.
PG  - S30-3
AB  - Isoenzyme-based approach was applied to compare Trichinella spiralis, T. britovi 
      and T. pseudospiralis species. Among 13 enzyme systems examined, esterase (EST), 
      malic enzyme (ME) and phosphoglucomutase (PGM) have been found as fully 
      diagnostic, with no common allele in species studied. Adenosine deaminase (ADA), 
      adenylate kinase (AK), hexokinase (HK), peptidase leucyl-alanine (PEP-C) and 
      fructose-bis-phosphatase (FBP) have been capable of distinguishing the two 
      species from resulting profiles. In addition, ADA, AK and PGM displayed the 
      enzyme expression in the lowest amounts of muscle larvae in systems tested (100 
      larvae/100 microliters of extracts). Based on allozyme data, T. pseudospiralis 
      has been found as the most distinct species within the group of taxa. Only a 
      subtle genetic variability was recorded for T. pseudospiralis in which solely 
      phosphoglucomutase exhibited variant patterns. In addition to the study of 
      reference isolates, T. spiralis from lowland fox in Eastern Slovakia has been 
      evidenced by use of genetic markers. This finding has proved that T. britovi is 
      not the exclusive species parasitizing in the sylvatic ecosystem of the Slovak 
      region.
FAU - Snabel, V
AU  - Snabel V
AD  - Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 040 01 Kosice, 
      Slovakia. snabel@saske.sk
FAU - Malakauskas, A
AU  - Malakauskas A
FAU - Dubinsky, P
AU  - Dubinsky P
FAU - Kapel, C M
AU  - Kapel CM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Parasite
JT  - Parasite (Paris, France)
JID - 9437094
RN  - 0 (Isoenzymes)
SB  - IM
MH  - Animals
MH  - Carnivora/parasitology
MH  - Foxes/parasitology
MH  - *Genetic Variation
MH  - Isoenzymes/*genetics
MH  - Phylogeny
MH  - Raccoons/parasitology
MH  - Swine/parasitology
MH  - Trichinella/*classification/enzymology/*genetics
MH  - Trichinella spiralis/classification/enzymology/genetics
EDAT- 2001/08/04 10:00
MHDA- 2001/10/05 10:01
CRDT- 2001/08/04 10:00
PHST- 2001/08/04 10:00 [pubmed]
PHST- 2001/10/05 10:01 [medline]
PHST- 2001/08/04 10:00 [entrez]
AID - 10.1051/parasite/200108s2030 [doi]
PST - ppublish
SO  - Parasite. 2001 Jun;8(2 Suppl):S30-3. doi: 10.1051/parasite/200108s2030.