PMID- 11484765
OWN - NLM
STAT- MEDLINE
DCOM- 20011212
LR  - 20190726
IS  - 0031-6768 (Print)
IS  - 0031-6768 (Linking)
VI  - 442
IP  - 3
DP  - 2001 Jun
TI  - Compensatory role of CaMKII on ICa and SR function during acidosis in rat 
      ventricular myocytes.
PG  - 353-61
AB  - It has been suggested that the activity of Ca2+/calmodulin-dependent protein 
      kinase II (CaMKII) increases during acidosis in cardiac muscle. Thus we have 
      investigated the role of CaMKII during acidosis by monitoring intracellular Ca2+ 
      (using fura-2) and ICa (using the perforated patch clamp technique) during 
      acidosis, in the absence and presence of the CaMKII inhibitor KN-93, in rat 
      isolated ventricular myocytes. In the absence of KN-93, acidosis (pH 6.5) 
      increased the amplitude of the fura-2 transient and prolonged its decay, but in 
      the presence of KN-93 acidosis did not alter the amplitude and prolonged the 
      decay more. In the absence of KN-93, acidosis increased the amplitude of the 
      caffeine-induced fura-2 transient but did not alter its amplitude in the presence 
      of KN-93. ICa did not change significantly during acidosis in the absence of 
      KN-93 but decreased during acidosis in the presence of KN-93. These results 
      suggest that activation of CaMKII during acidosis helps to compensate for the 
      direct inhibitory effects of acidosis on sarcoplasmic reticular Ca2+ uptake and 
      ICa.
FAU - Komukai, K
AU  - Komukai K
AD  - School of Biomedical Sciences, University of Leeds, UK.
FAU - Pascarel, C
AU  - Pascarel C
FAU - Orchard, C H
AU  - Orchard CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Pflugers Arch
JT  - Pflugers Archiv : European journal of physiology
JID - 0154720
RN  - 0 (Benzylamines)
RN  - 0 (Calcium Channels, L-Type)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Phosphodiesterase Inhibitors)
RN  - 0 (Sulfonamides)
RN  - 139298-40-1 (KN 93)
RN  - 3G6A5W338E (Caffeine)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - SY7Q814VUP (Calcium)
RN  - TSN3DL106G (Fura-2)
SB  - IM
MH  - Acidosis/*metabolism
MH  - Animals
MH  - Benzylamines/pharmacology
MH  - Caffeine/pharmacology
MH  - Calcium/*metabolism
MH  - Calcium Channels, L-Type/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Fluorescent Dyes
MH  - Fura-2
MH  - Heart Ventricles/cytology/metabolism
MH  - Hydrogen-Ion Concentration
MH  - Male
MH  - Muscle Fibers, Skeletal/*metabolism
MH  - Myocardium/cytology/*metabolism
MH  - Patch-Clamp Techniques
MH  - Phosphodiesterase Inhibitors/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Sarcoplasmic Reticulum/metabolism
MH  - Sulfonamides/pharmacology
EDAT- 2001/08/04 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/08/04 10:00
PHST- 2001/08/04 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/08/04 10:00 [entrez]
AID - 10.1007/s004240100549 [doi]
PST - ppublish
SO  - Pflugers Arch. 2001 Jun;442(3):353-61. doi: 10.1007/s004240100549.