PMID- 11485916
OWN - NLM
STAT- MEDLINE
DCOM- 20010906
LR  - 20220317
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 159
IP  - 2
DP  - 2001 Aug
TI  - Normal and malignant prostate epithelial cells differ in their response to 
      hepatocyte growth factor/scatter factor.
PG  - 579-90
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) promotes the proliferation, 
      differentiation, motility, and invasion of epithelial cells by binding to its 
      cell surface receptor, the Met tyrosine kinase. In the prostate, Met is expressed 
      predominantly by prostate epithelial cells (PrEC), whereas HGF/SF is synthesized 
      by prostate stromal cells (PrSC). Met is also expressed in localized and 
      metastatic prostate cancers. Our results show that PrECs in in vitro culture 
      maintain expression of Met at a level comparable to DU145 cancer cell expression. 
      HGF/SF secreted by PrSC stimulates tyrosine phosphorylation of the Met receptor. 
      In normal PrEC, HGF/SF causes growth inhibition, sustained phosphorylation of 
      mitogen-activated protein kinase, and increased CK18 expression consistent with 
      cell differentiation. In contrast, HGF/SF significantly stimulates the 
      proliferation of DU145 prostate cancer cells. HGF/SF in the conditioned medium of 
      PrSC specifically induces migration of both normal and malignant prostate 
      epithelial cells through MatriGel-coated Transwell filters. HGF/SF depletion 
      reduces cell migration by approximately 50%. The response of PrEC is specific for 
      HGF/SF since the other growth factors tested do not significantly affect growth 
      or migration of PrECs. These results support the in vivo importance of the 
      prostate stroma and specifically of HGF/SF as a unique stromal derived factor in 
      the development and progression of prostate cancer.
FAU - Gmyrek, G A
AU  - Gmyrek GA
AD  - Department of Pathology, Weill Medical College of Cornell University, 1300 York 
      Avenue, New York, NY 10021, USA.
FAU - Walburg, M
AU  - Walburg M
FAU - Webb, C P
AU  - Webb CP
FAU - Yu, H M
AU  - Yu HM
FAU - You, X
AU  - You X
FAU - Vaughan, E D
AU  - Vaughan ED
FAU - Vande Woude, G F
AU  - Vande Woude GF
FAU - Knudsen, B S
AU  - Knudsen BS
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Desmin)
RN  - 0 (Drug Combinations)
RN  - 0 (Laminin)
RN  - 0 (Proteoglycans)
RN  - 0 (Receptors, Androgen)
RN  - 0 (Vimentin)
RN  - 119978-18-6 (matrigel)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 68238-35-7 (Keratins)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Division/drug effects
MH  - Cell Line
MH  - Cell Movement/drug effects/physiology
MH  - Cells, Cultured
MH  - Collagen
MH  - Culture Media, Conditioned
MH  - Desmin/analysis/genetics
MH  - Dogs
MH  - Drug Combinations
MH  - Epithelial Cells/cytology/*drug effects/pathology
MH  - Hepatocyte Growth Factor/analysis/*genetics/*pharmacology
MH  - Humans
MH  - Immunohistochemistry
MH  - Keratins/analysis/genetics
MH  - Laminin
MH  - Male
MH  - Prostate/*cytology/drug effects/pathology
MH  - Prostatic Neoplasms/*pathology
MH  - Proteoglycans
MH  - Receptors, Androgen/analysis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
MH  - Vimentin/analysis/genetics
PMC - PMC1850543
EDAT- 2001/08/04 10:00
MHDA- 2001/09/08 10:01
PMCR- 2002/02/01
CRDT- 2001/08/04 10:00
PHST- 2001/08/04 10:00 [pubmed]
PHST- 2001/09/08 10:01 [medline]
PHST- 2001/08/04 10:00 [entrez]
PHST- 2002/02/01 00:00 [pmc-release]
AID - S0002-9440(10)61729-4 [pii]
AID - 2779 [pii]
AID - 10.1016/S0002-9440(10)61729-4 [doi]
PST - ppublish
SO  - Am J Pathol. 2001 Aug;159(2):579-90. doi: 10.1016/S0002-9440(10)61729-4.