PMID- 11486835
OWN - NLM
STAT- MEDLINE
DCOM- 20011207
LR  - 20210702
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 758
IP  - 2
DP  - 2001 Jul 15
TI  - High-performance liquid chromatography method for the quantification of 
      non-radiolabelled cinnamic compounds in analytes derived from human skin 
      absorption and metabolism experiments.
PG  - 249-64
AB  - An isocratic high-performance liquid chromatography method has been developed for 
      the quantification of the skin sensitisers trans-cinnamaldehyde and 
      trans-cinnamic alcohol, and their cinnamic metabolites. The relative standard 
      deviations (RSDs) between the gradients of eight sets of standard curves were 
      2.8, 3.1 and 1.9% for cinnamic alcohol, cinnamaldehyde and cinnamic acid, 
      respectively. Sample analytes were derived from two series of experiments: in 
      vitro full-thickness human skin absorption and metabolism studies and metabolism 
      studies using human skin homogenates, with non-radiolabelled cinnamic compounds. 
      Skin absorption and metabolism experiments were performed in the absence and 
      presence of the alcohol dehydrogenase inhibitor, pyrazole. Samples from 
      full-thickness skin absorption studies were analysed without extraction; cinnamic 
      compounds from within skin were extracted into methanolic solutions using newly 
      developed methods. The intra-assay RSDs ranged from 0.17 to 2.52% for cinnamic 
      alcohol, 0.24 to 9.14% for cinnamaldehyde and 0.26 to 6.43% for cinnamic acid. 
      The inter-assay RSDs for cinnamic alcohol, cinnamaldehyde and cinnamic acid, 
      respectively, as determined from n=20 HPLC runs, were 2.10, 4.16 and 2.26%.
FAU - Smith, C K
AU  - Smith CK
AD  - Division of Biomedical Sciences, Imperial College School of Medicine, South 
      Kensington, London, UK.
FAU - Cheung, C
AU  - Cheung C
FAU - Elahi, E N
AU  - Elahi EN
FAU - Hotchkiss, S A
AU  - Hotchkiss SA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 0 (Propanols)
RN  - 7864XYD3JJ (Acrolein)
RN  - SR60A3XG0F (cinnamaldehyde)
RN  - SS8YOP444F (cinnamyl alcohol)
SB  - IM
MH  - Acrolein/*analogs & derivatives/*analysis/pharmacokinetics
MH  - Adult
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Middle Aged
MH  - Propanols/*analysis/pharmacokinetics
MH  - Reproducibility of Results
MH  - *Skin Absorption
MH  - Spectrophotometry, Ultraviolet
EDAT- 2001/08/07 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/08/07 10:00
PHST- 2001/08/07 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/08/07 10:00 [entrez]
AID - 10.1016/s0378-4347(01)00184-0 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 2001 Jul 15;758(2):249-64. doi: 
      10.1016/s0378-4347(01)00184-0.