PMID- 11489166
OWN - NLM
STAT- MEDLINE
DCOM- 20011018
LR  - 20190915
IS  - 0141-9838 (Print)
IS  - 0141-9838 (Linking)
VI  - 23
IP  - 8
DP  - 2001 Aug
TI  - A glycoprotein from Schistosoma mansoni eggs binds non-antigen-specific 
      immunoglobulin E and releases interleukin-4 from human basophils.
PG  - 427-34
AB  - We have recently shown that soluble extracts from Schistosoma mansoni eggs (SmEA) 
      triggered basophils from nonsensitized donors to rapidly release interleukin 
      (IL)-4. Assuming that this mechanism might play a role in vivo in biasing the 
      immune response towards a Th2 phenotype, we determined basic properties of the 
      IL-4-inducing activity contained in SmEA. Sensitivity to pepsin digestion 
      indicated protein nature. Binding to and specific elution from Concanavalin 
      A-sepharose suggested that this protein contains mannose residues, thus being a 
      glycoprotein. The IL-4-inducing activity was stable for 30 min at room 
      temperature towards shifting the pH between 3 and 10. When incubated at 100 
      degrees C, it was stable at pH 3, but less stable at neutral and alkaline pH. 
      Electroelution from an SDS-PAGE gel indicated an apparent molecular weight of the 
      IL-4-inducing activity between 31 and 66 kDa. Although binding to purified human 
      immunoglobulin E (IgE) and activating basophils IgE-dependently, SmEA appears to 
      activate basophils in a non-antigen-specific way, since the cells were purified 
      from noninfected donors. Because the IL-4-inducing activity was found to be 
      released from eggs, it could be an important factor in the environment of the 
      eggs skewing the immune response towards the Th2 phenotype.
FAU - Haisch, K
AU  - Haisch K
AD  - Department of Microbiology, SUNY at Buffalo, Buffalo, NY, USA.
FAU - Schramm, G
AU  - Schramm G
FAU - Falcone, F H
AU  - Falcone FH
FAU - Alexander, C
AU  - Alexander C
FAU - Schlaak, M
AU  - Schlaak M
FAU - Haas, H
AU  - Haas H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Parasite Immunol
JT  - Parasite immunology
JID - 7910948
RN  - 0 (Glycoproteins)
RN  - 0 (Helminth Proteins)
RN  - 11028-71-0 (Concanavalin A)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Basophils/*metabolism
MH  - Concanavalin A/metabolism
MH  - Glycoproteins/*metabolism
MH  - Helminth Proteins/*metabolism
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Immunoglobulin E/*metabolism
MH  - Interleukin-4/*biosynthesis
MH  - Molecular Weight
MH  - Ovum/chemistry/immunology
MH  - Schistosoma mansoni/chemistry/*immunology
MH  - Temperature
EDAT- 2001/08/08 10:00
MHDA- 2001/10/19 10:01
CRDT- 2001/08/08 10:00
PHST- 2001/08/08 10:00 [pubmed]
PHST- 2001/10/19 10:01 [medline]
PHST- 2001/08/08 10:00 [entrez]
AID - pim392 [pii]
AID - 10.1046/j.1365-3024.2001.00392.x [doi]
PST - ppublish
SO  - Parasite Immunol. 2001 Aug;23(8):427-34. doi: 10.1046/j.1365-3024.2001.00392.x.