PMID- 11489182
OWN - NLM
STAT- MEDLINE
DCOM- 20010927
LR  - 20240109
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 27
IP  - 1
DP  - 2001 Jul
TI  - Integration of the FISH pachytene and genetic maps of Medicago truncatula.
PG  - 49-58
AB  - A molecular cytogenetic map of Medicago truncatula (2n = 2x = 16) was constructed 
      on the basis of a pachytene DAPI karyogram. Chromosomes at this meiotic prophase 
      stage are 20 times longer than at mitotic metaphase, and display a well 
      differentiated pattern of brightly fluorescing heterochromatin segments. We 
      describe here a pachytene karyogram in which all chromosomes can be identified 
      based on chromosome length, centromere position, heterochromatin patterns, and 
      the positions of three repetitive sequences (5S rDNA, 45S rDNA and the MtR1 
      tandem repeat), visualized by fluorescence in situ hybridization (FISH). We 
      determined the correlation between genetic linkage groups and chromosomes by FISH 
      mapping of bacterial artificial chromosome (BAC) clones, with two to five BACs 
      per linkage group. In the cytogenetic map, chromosomes were numbered according to 
      their corresponding linkage groups. We determined the relative positions of the 
      20 BACs and three repetitive sequences on the pachytene chromosomes, and compared 
      the genetic and cytological distances between markers. The mapping resolution was 
      determined in a euchromatic part of chromosome 5 by comparing the cytological 
      distances between FISH signals of clones of a BAC contig with their corresponding 
      physical distance, and showed that resolution in this region is about 60 kb. The 
      establishment of this FISH pachytene karyotype, with a far better mapping 
      resolution and detection sensitivity compared to those in the highly condensed 
      mitotic metaphase complements, has created the basis for the integration of 
      molecular, genetic and cytogenetic maps in M. truncatula.
FAU - Kulikova, O
AU  - Kulikova O
AD  - Wageningen University, Department of Plant Sciences, Laboratory of Molecular 
      Biology, the Netherlands. olga.kulikova@mac.mb.wau.nl
FAU - Gualtieri, G
AU  - Gualtieri G
FAU - Geurts, R
AU  - Geurts R
FAU - Kim, D J
AU  - Kim DJ
FAU - Cook, D
AU  - Cook D
FAU - Huguet, T
AU  - Huguet T
FAU - de Jong, J H
AU  - de Jong JH
FAU - Fransz, P F
AU  - Fransz PF
FAU - Bisseling, T
AU  - Bisseling T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (DNA Primers)
SB  - IM
MH  - Base Sequence
MH  - *Chromosome Mapping
MH  - Chromosomes, Artificial, Bacterial
MH  - DNA Primers
MH  - Genetic Linkage
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Medicago sativa/*genetics
EDAT- 2001/08/08 10:00
MHDA- 2001/09/28 10:01
CRDT- 2001/08/08 10:00
PHST- 2001/08/08 10:00 [pubmed]
PHST- 2001/09/28 10:01 [medline]
PHST- 2001/08/08 10:00 [entrez]
AID - tpj1057 [pii]
AID - 10.1046/j.1365-313x.2001.01057.x [doi]
PST - ppublish
SO  - Plant J. 2001 Jul;27(1):49-58. doi: 10.1046/j.1365-313x.2001.01057.x.