PMID- 11489260
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 910
IP  - 1-2
DP  - 2001 Aug 10
TI  - Altered gene expression in cerebral capillaries of stroke-prone spontaneously 
      hypertensive rats.
PG  - 106-15
AB  - Stroke-prone spontaneously hypertensive rats (SHRSP) are a well-characterized, 
      genetic model for stroke. We showed earlier that the structure and function of 
      the tight junctions in SHRSP blood-brain barrier endothelial cells is disturbed 
      prior to stroke. To investigate the molecular events leading to endothelial 
      dysfunction in SHRSP cerebral capillaries, we carried out suppression subtractive 
      hybridization (SSH) in combination with a cDNA filter screening step. We 
      identified two cDNA fragments that were upregulated in SHRSP, compared to 
      stroke-resistant spontaneously hypertensive rats (SHR), and found open reading 
      frames of 133 and 138 amino acids, respectively. These peptides did not match any 
      known proteins in public databases. A third upregulated SHRSP cDNA fragment was 
      identified as the rat sulfonylurea receptor 2B (SUR2B). We also isolated and 
      cloned the cDNA of the rat homologue for the mouse G-protein signaling 5 (RGS5) 
      regulator. This regulator was downregulated in SHRSP. We used in situ 
      hybridization to show that rat RGS5 is expressed in the brain capillary 
      endothelium and in the choroid plexus. Our findings may lead to the 
      identification of new stroke-related genes.
FAU - Kirsch, T
AU  - Kirsch T
AD  - Max-Delbruck-Center for Molecular Medicine, Berlin, Germany. 
      tkirsch@mdc-berlin.de
FAU - Wellner, M
AU  - Wellner M
FAU - Luft, F C
AU  - Luft FC
FAU - Haller, H
AU  - Haller H
FAU - Lippoldt, A
AU  - Lippoldt A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Abcc9 protein, mouse)
RN  - 0 (DNA, Complementary)
RN  - 0 (Peptide Fragments)
RN  - 0 (Potassium Channels)
RN  - 0 (Potassium Channels, Inwardly Rectifying)
RN  - 0 (RGS Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Drug)
RN  - 0 (Rgs5 protein, rat)
RN  - 0 (Sulfonylurea Receptors)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - *ATP-Binding Cassette Transporters
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blood-Brain Barrier/genetics
MH  - Causality
MH  - Cerebral Arteries/*metabolism/pathology/physiopathology
MH  - DNA, Complementary/*genetics/isolation & purification/metabolism
MH  - Endothelium, Vascular/*metabolism/pathology/physiopathology
MH  - GTP-Binding Proteins/metabolism
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Hypertension/complications/genetics/*metabolism
MH  - In Situ Hybridization/methods
MH  - Molecular Sequence Data
MH  - Peptide Fragments/genetics
MH  - Potassium Channels/genetics/metabolism
MH  - *Potassium Channels, Inwardly Rectifying
MH  - RGS Proteins/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Inbred SHR/abnormalities/genetics/*metabolism
MH  - Receptors, Drug/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Nucleic Acid
MH  - Signal Transduction/physiology
MH  - Stroke/genetics/*metabolism/physiopathology
MH  - Sulfonylurea Receptors
EDAT- 2001/08/08 10:00
MHDA- 2001/10/26 10:01
CRDT- 2001/08/08 10:00
PHST- 2001/08/08 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/08/08 10:00 [entrez]
AID - S0006-8993(01)02670-1 [pii]
AID - 10.1016/s0006-8993(01)02670-1 [doi]
PST - ppublish
SO  - Brain Res. 2001 Aug 10;910(1-2):106-15. doi: 10.1016/s0006-8993(01)02670-1.