PMID- 11489349
OWN - NLM
STAT- MEDLINE
DCOM- 20010913
LR  - 20190719
IS  - 0361-9230 (Print)
IS  - 0361-9230 (Linking)
VI  - 55
IP  - 3
DP  - 2001 Jun
TI  - Optic nerve regeneration within artificial Schwann cell graft in the adult rat.
PG  - 409-19
AB  - We investigate whether an artificial graft made by cultured Schwann cell, 
      extracellular matrix (ECM) and trophic factors can provide the environment for 
      the regeneration of retinal ganglion cell (RGC) axons in adult rats. Six kinds of 
      artificial grafts were used: ECM (control); ECM and Schwann cells; ECM, Schwann 
      cells and either nerve growth factor, brain-derived neurotrophic factor (BDNF) 
      and neurotrophin-4 (NT-4); ECM, Schwann cells, BDNF and NT-4, combined with 
      intravitreal injection of BDNF. The grafts were transplanted onto the transected 
      optic nerve. RGC regeneration was evaluated by dil retrograde labeling, 
      immunohistochemistry, and electron microscopy at 3 weeks post-operation. The 
      degree of dil labeled RGC was approximately 2% for ECM alone, and 10% for ECM and 
      Schwann cells (p < 0.01). The labeling increased to approximately 20% by 
      administration of neurotrophins. The addition of intravitreous BDNF injection 
      resulted in highest labeling percentage of 30%. Immunohistochemical study showed 
      that axons were association with GAP-43 and cell adhesion molecules. Neurotrophin 
      receptors (Trk-A and Trk-B) were detected in nerve fibers both in the retina and 
      in the graft. Remyelination was seen by electron microscopic observation. These 
      results demonstrate that the regeneration of RGC axons is induced with the use of 
      cultured Schwann cells and ECM as promoting factors for regrowth. The degree of 
      regeneration was significantly increased by neurotrophins in the grafts and in 
      the vitreous.
FAU - Negishi, H
AU  - Negishi H
AD  - Department of Ophthalmology and Visual Science (D1), Graduate School of Medicine, 
      Chiba University, Chuo-ku, Chiba, Japan. negishi@ophthalm.m.chiba-u.ac.jp
FAU - Dezawa, M
AU  - Dezawa M
FAU - Oshitari, T
AU  - Oshitari T
FAU - Adachi-Usami, E
AU  - Adachi-Usami E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Brain Res Bull
JT  - Brain research bulletin
JID - 7605818
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
SB  - IM
MH  - Animals
MH  - Axons/physiology
MH  - Cell Survival/drug effects
MH  - Immunohistochemistry
MH  - Male
MH  - Microscopy, Electron
MH  - Microscopy, Immunoelectron
MH  - Nerve Growth Factors/pharmacology
MH  - *Nerve Regeneration
MH  - Nerve Tissue Proteins/metabolism
MH  - Optic Nerve/pathology/*physiopathology/*surgery
MH  - Rats
MH  - Rats, Wistar
MH  - Retinal Ganglion Cells/physiology
MH  - Schwann Cells/physiology/*transplantation
EDAT- 2001/08/08 10:00
MHDA- 2001/09/14 10:01
CRDT- 2001/08/08 10:00
PHST- 2001/08/08 10:00 [pubmed]
PHST- 2001/09/14 10:01 [medline]
PHST- 2001/08/08 10:00 [entrez]
AID - S0361-9230(01)00534-2 [pii]
AID - 10.1016/s0361-9230(01)00534-2 [doi]
PST - ppublish
SO  - Brain Res Bull. 2001 Jun;55(3):409-19. doi: 10.1016/s0361-9230(01)00534-2.