PMID- 11489664
OWN - NLM
STAT- MEDLINE
DCOM- 20011101
LR  - 20191105
IS  - 1056-8719 (Print)
IS  - 1056-8719 (Linking)
VI  - 45
IP  - 1
DP  - 2001 Jan-Feb
TI  - A flowcytometric method for evaluation of acid secretion from isolated rat 
      gastric mucosal cells.
PG  - 47-53
AB  - Studies on the gastric proton pump are mostly performed on the H(+), K(+)-ATPase 
      enzyme in the microsomal preparation or by aminopyrine accumulation in the 
      gastric parietal cells. H(+), K(+)-ATPase activity is estimated by both 
      spectrophotometric and fluorimetric methods. In the present study, quenching or 
      augmentation in acridine orange (AO) fluorescence was monitored on a 
      flowcytometer in rat gastric mucosal cells. Rat gastric mucosal cells were 
      isolated by the standard pronase--EDTA method. The effect of oleic acid, a proton 
      pump inhibitory was evaluated on gastric parietal cell activity and was compared 
      with its effect on proton transport, H(+), K(+)-ATPase, and p-nitrophenyl 
      phosphatase (p-NPPase) activity in gastric microsomes. In addition, the effect of 
      histamine and carbachol, gastric acid release inducers, was also investigated by 
      flowcytometry in isolated parietal cells. Histamine and carbachol, in a 
      dose-dependent manner, stimulated acid release from isolated gastric cells. Oleic 
      acid also dose-dependently inhibited the basal and stimulated acid release from 
      the cells, as well as in all three enzyme preparations associated with gastric 
      proton pump activity. Thus, the results suggest that flowcytometric method might 
      be used to study basal, as well as stimulated, proton pump activity in isolated 
      gastric parietal cells.
FAU - Dixit, C
AU  - Dixit C
AD  - Division of Pharmacology, Central Drug Research Institute, Lucknow 226001, India.
FAU - Dikshit, M
AU  - Dikshit M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pharmacol Toxicol Methods
JT  - Journal of pharmacological and toxicological methods
JID - 9206091
RN  - 0 (Histamine Antagonists)
RN  - 0 (Proton Pumps)
RN  - 2UMI9U37CP (Oleic Acid)
RN  - 8Y164V895Y (Carbachol)
RN  - EC 3.1.3.41 (4-Nitrophenylphosphatase)
RN  - EC 3.6.3.10 (H(+)-K(+)-Exchanging ATPase)
SB  - IM
MH  - 4-Nitrophenylphosphatase/metabolism
MH  - Animals
MH  - Carbachol/antagonists & inhibitors
MH  - Cells, Cultured
MH  - Flow Cytometry/*methods
MH  - Gastric Acid/*metabolism
MH  - Gastric Mucosa/cytology/*drug effects/metabolism
MH  - H(+)-K(+)-Exchanging ATPase/metabolism
MH  - Histamine Antagonists
MH  - In Vitro Techniques
MH  - Microsomes
MH  - Oleic Acid/antagonists & inhibitors
MH  - Parietal Cells, Gastric/metabolism
MH  - Proton Pumps/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stomach/enzymology
EDAT- 2001/08/08 10:00
MHDA- 2001/11/03 10:01
CRDT- 2001/08/08 10:00
PHST- 2001/08/08 10:00 [pubmed]
PHST- 2001/11/03 10:01 [medline]
PHST- 2001/08/08 10:00 [entrez]
AID - S1056-8719(01)00125-3 [pii]
AID - 10.1016/s1056-8719(01)00125-3 [doi]
PST - ppublish
SO  - J Pharmacol Toxicol Methods. 2001 Jan-Feb;45(1):47-53. doi: 
      10.1016/s1056-8719(01)00125-3.