PMID- 11489827
OWN - NLM
STAT- MEDLINE
DCOM- 20011018
LR  - 20141120
IS  - 1078-0432 (Print)
IS  - 1078-0432 (Linking)
VI  - 7
IP  - 8
DP  - 2001 Aug
TI  - Fluorescence in situ hybridization evaluation of c-erbB-2 gene amplification and 
      chromosomal anomalies in bladder cancer.
PG  - 2463-7
AB  - Oncogene amplification and chromosomal anomalies are found in many solid tumors 
      and are often associated with aggressiveness of cancer. We evaluated the 
      frequency and the role of c-erbB-2 gene amplification, relative increase in 
      c-erbB-2 gene copy number, and gain of chromosome 17 in bladder cancer. A total 
      of 29 bladder cancer specimens were examined using fluorescence in situ 
      hybridization (FISH). Dual labeling hybridization with a directly labeled 
      centromere probe for chromosome 17 together with a probe for the c-erbB-2 locus 
      was performed. c-erbB-2 gene amplification was found in 3.4% (1 of 29) of 
      specimens. Relative increase in c-erbB-2 gene copy number was found in 41.4% (12 
      of 29) of specimens and was significantly associated with tumor grade (P = 0.044 
      by Fisher's exact test). Gain of chromosome 17 was identified in 65.5% (19 of 29) 
      of specimens and was significantly associated with tumor grade (P = 0.002 by 
      Fisher's exact test) and tumor stage (P = 0.003 by Fisher's exact test). Our 
      results suggest that c-erbB-2 gene amplification, relative increase in c-erbB-2 
      gene copy number, and gain of chromosome 17 may play important roles in the 
      development and progression of bladder cancers. Moreover, the use of c-erbB-2 
      amplification, relative increase in c-erbB-2 gene copy number, and gain of 
      chromosome 17 using FISH, together with tumor grade and stage, may provide a more 
      useful clinical indicator in bladder cancer.
FAU - Ohta, J I
AU  - Ohta JI
AD  - Departments of Urology, Yokohama City University School of Medicine, 3-9 Fukuura, 
      Kanazawa-ku, Yokohama 236-0004, Japan.
FAU - Miyoshi, Y
AU  - Miyoshi Y
FAU - Uemura, H
AU  - Uemura H
FAU - Fujinami, K
AU  - Fujinami K
FAU - Mikata, K
AU  - Mikata K
FAU - Hosaka, M
AU  - Hosaka M
FAU - Tokita, Y
AU  - Tokita Y
FAU - Kubota, Y
AU  - Kubota Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Cancer Res
JT  - Clinical cancer research : an official journal of the American Association for 
      Cancer Research
JID - 9502500
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 17/genetics
MH  - *Gene Amplification
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Receptor, ErbB-2/*genetics
MH  - Urinary Bladder Neoplasms/*genetics/pathology
EDAT- 2001/08/08 10:00
MHDA- 2001/10/19 10:01
CRDT- 2001/08/08 10:00
PHST- 2001/08/08 10:00 [pubmed]
PHST- 2001/10/19 10:01 [medline]
PHST- 2001/08/08 10:00 [entrez]
PST - ppublish
SO  - Clin Cancer Res. 2001 Aug;7(8):2463-7.