PMID- 11491544
OWN - NLM
STAT- MEDLINE
DCOM- 20011211
LR  - 20161018
IS  - 0730-7268 (Print)
IS  - 0730-7268 (Linking)
VI  - 20
IP  - 8
DP  - 2001 Aug
TI  - Dose- and time-dependent formation of biliary benzo[a]pyrene metabolites in the 
      marine flatfish dab (Limanda limanda).
PG  - 1641-7
AB  - Polycyclic aromatic hydrocarbons (PAHs) are abundant pollutants, and many PAHs 
      are carcinogenic, but only after metabolic activation. Benzo[a]pyrene (BaP) is 
      among the most carcinogenic PAHs. The dose and time response of two enzymes 
      involved in BaP metabolism and the amounts of BaP metabolites excreted into the 
      bile were evaluated in an experiment with dab (Limanda limanda). Ninety dab were 
      exposed orally to one of five doses of BaP (0, 0.08, 0.4, 2, or 10 mg/kg) and 
      sampled at 3, 6, or 12 d after exposure. None of the doses studied caused 
      significant induction of either microsomal ethoxyresorufin-O-deethylase (EROD). 
      which reflects cytochrome P450 1A (CYP1A) activity, or cytosolic 
      glutathione-S-transferase activity (GST). Concentrations of biliary BaP 
      metabolites significantly increased with dose and significantly decreased with 
      time after exposure. It is concluded that biliary BaP metabolites provide a much 
      more sensitive method than EROD (CYP1A) or GST activity to monitor recent 
      exposure to PAHs in dab.
FAU - van Schanke, A
AU  - van Schanke A
AD  - Netherlands Institute for Sea Research, Department of Marine Biogeochemistry and 
      Toxicology, Den Burg Texel. a.vanschanke@azu.nl
FAU - Holtz, F
AU  - Holtz F
FAU - van der Meer, J P
AU  - van der Meer JP
FAU - Boon, J P
AU  - Boon JP
FAU - Ariese, F
AU  - Ariese F
FAU - Stroomberg, G
AU  - Stroomberg G
FAU - van den Berg, M
AU  - van den Berg M
FAU - Everaarts, J M
AU  - Everaarts JM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Environ Toxicol Chem
JT  - Environmental toxicology and chemistry
JID - 8308958
RN  - 0 (Biomarkers)
RN  - 0 (Carcinogens)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Benzo(a)pyrene/chemistry/*metabolism/pharmacokinetics
MH  - Bile/chemistry
MH  - Biomarkers/analysis
MH  - Carcinogens/chemistry/*metabolism/pharmacokinetics
MH  - Cytochrome P-450 CYP1A1/analysis/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Environmental Exposure
MH  - Environmental Monitoring
MH  - Flatfishes/*physiology
MH  - Glutathione Transferase/analysis/pharmacology
MH  - Kinetics
MH  - Sensitivity and Specificity
EDAT- 2001/08/09 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/08/09 10:00
PHST- 2001/08/09 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/08/09 10:00 [entrez]
PST - ppublish
SO  - Environ Toxicol Chem. 2001 Aug;20(8):1641-7.