PMID- 11493263
OWN - NLM
STAT- MEDLINE
DCOM- 20010913
LR  - 20190915
IS  - 0268-9146 (Print)
IS  - 0268-9146 (Linking)
VI  - 32
IP  - 3
DP  - 2001 Jun
TI  - Chromosome assignment of six dog genes by FISH, and correlation with dog-human 
      Zoo-FISH data.
PG  - 148-51
AB  - Cross-species chromosome painting analyses have recently demonstrated the 
      presence of regions of conserved synteny between the human and domestic dog 
      genomes, aiding the search for candidate genes for inherited traits. Concerted 
      efforts to subchromosomally assign substantial numbers of dog gene sequences are 
      now needed in order to refine these comparative data, both in terms of marker 
      density and resolution. We have developed novel PCR markers representing three 
      dog genes (ALB, FOS, HNRPA2B1) for which no sequence or mapping data were 
      previously available, to our knowledge. These, in addition to three gene markers 
      previously described (ALDOA, RPE65, VCAM1), were used to isolate and 
      chromosomally assign corresponding large insert genomic clones by fluorescence in 
      situ hybridization (FISH). Chromosome assignments for these six dog genes are 
      discussed in terms of those of the human orthologues, and correlated with 
      existing comparative mapping information, identifying one apparent exception to 
      existing Zoo-FISH data, and aiding refinement of the boundaries of conserved 
      chromosome segments in both genomes.
FAU - Thomas, R
AU  - Thomas R
AD  - Genetics Section, Centre for Preventive Medicine, Animal Health Trust, Lanwades 
      Park, Kentford, Newmarket, Suffolk, CB8 7UU, UK. rthomas@hgmp.mrc.ac.uk
FAU - Breen, M
AU  - Breen M
FAU - Binns, M M
AU  - Binns MM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Anim Genet
JT  - Animal genetics
JID - 8605704
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - *Chromosome Mapping
MH  - Chromosome Painting/methods
MH  - Dogs/*genetics
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Polymerase Chain Reaction
EDAT- 2001/08/09 10:00
MHDA- 2001/09/14 10:01
CRDT- 2001/08/09 10:00
PHST- 2001/08/09 10:00 [pubmed]
PHST- 2001/09/14 10:01 [medline]
PHST- 2001/08/09 10:00 [entrez]
AID - age731 [pii]
AID - 10.1046/j.1365-2052.2001.00731.x [doi]
PST - ppublish
SO  - Anim Genet. 2001 Jun;32(3):148-51. doi: 10.1046/j.1365-2052.2001.00731.x.