PMID- 11493312
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20220408
IS  - 0906-6705 (Print)
IS  - 0906-6705 (Linking)
VI  - 10
IP  - 4
DP  - 2001 Aug
TI  - Psoriasis scales contain C5a as the predominant chemotaxin for monocyte-derived 
      dendritic cells.
PG  - 238-45
AB  - Dendritic cells seem to be of major importance for the pathogenesis of psoriasis. 
      They are increased in number in lesional psoriatic skin which is thought to be 
      due to an increased influx from the peripheral blood regulated by chemotaxins. 
      Using a biological/biochemical approach we have addressed the question whether 
      psoriasis scale extracts contain proteinaceous chemotaxins for dendritic cells. 
      Human monocytes differentiated into dendritic cells by culture with GM-CSF and 
      IL-4 (MoDC) served as responder cells. Chemotactic activity for MoDC was purified 
      by several HPLC-steps. The results of our study show that C5a/C5adesarg is the 
      major chemotactic peptide for MoDC in psoriasis scale extracts. In comparison to 
      other stimuli such as fMLP or monocyte chemotactic peptide 1 (MCP-1) C5a proved 
      to be a most potent and efficient chemotaxin for MoDC. C5a co-eluted with 
      MRP14/calgranulin B which is present in large amounts in psoriasis scale extracts 
      as identified by amino acid sequencing. However, MRP14/calgranulin B did not 
      possess any chemotactic activity for MoDC. Our results provide evidence that 
      C5a/C5adesarg although not specific for dendritic cells seems to be the major 
      chemoattractant for these cells in lesional psoriasis skin.
FAU - Mrowietz, U
AU  - Mrowietz U
AD  - Dept of Dermatology, University of Kiel, Schittenhelmstr. 7, 24105 Kiel, Germany. 
      umrowietz@dermatology.uni-kiel.de
FAU - Koch, W A
AU  - Koch WA
FAU - Zhu, K
AU  - Zhu K
FAU - Wiedow, O
AU  - Wiedow O
FAU - Bartels, J
AU  - Bartels J
FAU - Christophers, E
AU  - Christophers E
FAU - Schroder, J M
AU  - Schroder JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Exp Dermatol
JT  - Experimental dermatology
JID - 9301549
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Calgranulin B)
RN  - 0 (Chemotactic Factors)
RN  - 0 (S100 Proteins)
RN  - 80295-54-1 (Complement C5a)
SB  - IM
MH  - Amino Acid Sequence/genetics
MH  - Antigens, Differentiation/genetics/isolation & purification/physiology
MH  - Calcium-Binding Proteins/genetics/isolation & purification/physiology
MH  - Calgranulin B
MH  - Cell Division
MH  - Chemotactic Factors/*metabolism
MH  - Chemotaxis/physiology
MH  - Complement C5a/*metabolism
MH  - Dendritic Cells/*cytology/*physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Monocytes/*cytology
MH  - Neutrophils/chemistry
MH  - Psoriasis/*metabolism/pathology
MH  - S100 Proteins/genetics/isolation & purification/physiology
EDAT- 2001/08/09 10:00
MHDA- 2001/10/26 10:01
CRDT- 2001/08/09 10:00
PHST- 2001/08/09 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/08/09 10:00 [entrez]
AID - exd100403 [pii]
AID - 10.1034/j.1600-0625.2001.100403.x [doi]
PST - ppublish
SO  - Exp Dermatol. 2001 Aug;10(4):238-45. doi: 10.1034/j.1600-0625.2001.100403.x.