PMID- 11493755
OWN - NLM
STAT- MEDLINE
DCOM- 20010906
LR  - 20180213
IS  - 1015-2008 (Print)
IS  - 1015-2008 (Linking)
VI  - 68
IP  - 6
DP  - 2000
TI  - Analyses of matrix metalloproteinases and their inhibitors in cyst fluid of 
      serous ovarian tumors.
PG  - 239-44
AB  - OBJECTIVES: Expression of matrix metalloproteinases (MMPs) and tissue inhibitors 
      of MMPs (TIMPs) in serous tumors of the ovary was investigated to determine 
      whether and how these proteolytic enzymes are associated with the progression of 
      these tumors. METHODS: Cyst fluid of 24 serous ovarian tumors (8 adenocarcinomas, 
      2 borderline tumors and 14 adenomas) was analyzed using gelatin/casein zymography 
      and enzyme-linked immunosorbent assay. RESULTS: Concentrations of MMP-9 and MMP-2 
      were statistically higher in serous adenocarcinomas than in serous adenomas (p < 
      0.01, p < 0.05, respectively), while the concentrations of TIMP-1 and TIMP-2 
      showed no significant difference between adenocarcinomas and adenomas. The molar 
      ratio of TIMP-2/MMP-2 was lower in adenocarcinomas than in adenomas (p < 0.05). 
      With gelatin zymography, the MMP-9 band was detected in all serous 
      adenocarcinomas, but only in 8 of 14 serous adenomas (p = 0.05). Using casein 
      zymography, MMP-7 was more frequently detected in serous adenocarcinomas (7/8) 
      than in serous adenomas (4/14; p < 0.05). CONCLUSIONS: These observations 
      indicate that matriolytic enzymes such as MMP-2, MMP-7 and MMP-9 are secreted 
      into cyst fluid from serous adenocarcinoma tissues. In part, the aggressive 
      invasion of serous carcinoma cells may be explained by the expression of 
      matriolytic enzymes.
CI  - Copyright 2001 S. Karger AG, Basel
FAU - Furuya, M
AU  - Furuya M
AD  - Department of Pathology, Hokkaido University School of Medicine, N-15, W-7, 
      Kita-ku, Sapporo 060-8638, Japan.
FAU - Ishikura, H
AU  - Ishikura H
FAU - Ogawa, Y
AU  - Ogawa Y
FAU - Kawarada, Y
AU  - Kawarada Y
FAU - Shibata, M
AU  - Shibata M
FAU - Fujimoto, S
AU  - Fujimoto S
FAU - Yoshiki, T
AU  - Yoshiki T
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Pathobiology
JT  - Pathobiology : journal of immunopathology, molecular and cellular biology
JID - 9007504
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Adenoma/*enzymology/pathology/surgery
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Blotting, Western
MH  - Cyst Fluid/chemistry/*enzymology
MH  - Cystadenocarcinoma, Serous/*enzymology/pathology/surgery
MH  - Disease Progression
MH  - Electrophoresis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Matrix Metalloproteinase Inhibitors
MH  - Matrix Metalloproteinases/analysis/classification/*metabolism
MH  - Middle Aged
MH  - Ovarian Neoplasms/*enzymology/pathology/surgery
MH  - Tissue Inhibitor of Metalloproteinases/analysis/metabolism
EDAT- 2001/08/09 10:00
MHDA- 2001/09/08 10:01
CRDT- 2001/08/09 10:00
PHST- 2001/08/09 10:00 [pubmed]
PHST- 2001/09/08 10:01 [medline]
PHST- 2001/08/09 10:00 [entrez]
AID - pat68239 [pii]
AID - 10.1159/000055932 [doi]
PST - ppublish
SO  - Pathobiology. 2000;68(6):239-44. doi: 10.1159/000055932.