PMID- 11494118
OWN - NLM
STAT- MEDLINE
DCOM- 20011004
LR  - 20240412
IS  - 1522-8002 (Print)
IS  - 1476-5586 (Electronic)
IS  - 1476-5586 (Linking)
VI  - 3
IP  - 3
DP  - 2001 May-Jun
TI  - Chromosome 7 aneusomy. A marker for metastatic melanoma? Expression of the 
      epidermal growth factor receptor gene and chromosome 7 aneusomy in nevi, primary 
      malignant melanomas and metastases.
PG  - 245-54
AB  - Receptor tyrosine kinases such as the epidermal growth factor receptor (EGFR) 
      play an important role in a variety of malignant neoplasias, making the search 
      for aberrations in the relevant chromosomes an important issue. Differential 
      expression of the EGFR gene was investigated by reverse transcriptase (RT)-PCR on 
      tissue samples of normal skin, nevi, primary melanomas, and melanoma metastases. 
      The EGFR gene is located on chromosome 7p12.3-p12.1. To determine the number of 
      chromosomes 7 in cell nuclei of the mentioned tissue samples we performed 
      fluorescence in situ hybridization (FISH) on touch preparations, using a DNA 
      probe that hybridizes specifically to the centromeric region of chromosome 7. 
      Additionally, chromosome 7 number in interphase nuclei was determined in 
      short-term primary cell cultures of nevi, primary melanomas, and metastases. The 
      highest EGFR gene expression frequency was found in melanoma metastases. By FISH 
      we detected the highest fraction of cell nuclei with more than two chromosomes 7 
      in the group of metastases. Our results suggest that overexpression of the EGFR 
      gene might play an important role in metastasis of malignant melanoma. This is 
      well reflected by polysomy 7, possibly accounting for an increased EGFR gene copy 
      number.
FAU - Udart, M
AU  - Udart M
AD  - Department of Dermatology, University of Ulm, Ulm, Germany. 
      martin.udart@medizin.uni-ulm.de
FAU - Utikal, J
AU  - Utikal J
FAU - Krahn, G M
AU  - Krahn GM
FAU - Peter, R U
AU  - Peter RU
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neoplasia
JT  - Neoplasia (New York, N.Y.)
JID - 100886622
RN  - 0 (DNA Probes)
RN  - 0 (Genetic Markers)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - *Aneuploidy
MH  - Chromosomes, Human, Pair 7/*genetics
MH  - DNA Probes/analysis
MH  - ErbB Receptors/*metabolism
MH  - Gene Expression
MH  - Genes, erbB-1/physiology
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Melanoma/genetics/*metabolism/pathology
MH  - Neoplasm Metastasis/genetics/pathology
MH  - Nevus/genetics/*metabolism/pathology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Staining and Labeling
MH  - Tumor Cells, Cultured
PMC - PMC1505589
EDAT- 2001/08/09 10:00
MHDA- 2001/10/05 10:01
PMCR- 2001/05/01
CRDT- 2001/08/09 10:00
PHST- 2000/10/30 00:00 [received]
PHST- 2001/01/15 00:00 [accepted]
PHST- 2001/08/09 10:00 [pubmed]
PHST- 2001/10/05 10:01 [medline]
PHST- 2001/08/09 10:00 [entrez]
PHST- 2001/05/01 00:00 [pmc-release]
AID - 0156a [pii]
AID - 10.1038/sj/neo/7900156 [doi]
PST - ppublish
SO  - Neoplasia. 2001 May-Jun;3(3):245-54. doi: 10.1038/sj/neo/7900156.