PMID- 11496239
OWN - NLM
STAT- MEDLINE
DCOM- 20010906
LR  - 20171116
IS  - 0091-6749 (Print)
IS  - 0091-6749 (Linking)
VI  - 108
IP  - 2
DP  - 2001 Aug
TI  - Inhibition of IgE production in vitro by intact and fragmented intravenous 
      immunoglobulin.
PG  - 229-34
AB  - BACKGROUND: Intravenous immunoglobulin (IVIG) has been shown to suppress Ig 
      production both in vivo and in vitro. We have previously found that IVIG inhibits 
      IgE synthesis in human tonsillar B cells stimulated with IL-4 and anti-CD40 
      antibody. OBJECTIVE: The purpose of this study was to further clarify the 
      mechanism behind the inhibition of IgE production by IVIG through comparing the 
      effects of intact whole molecular IVIG and the F(ab')(2) or Fc fragments of IVIG. 
      METHODS: Human B lymphocytes were purified from tonsils. Cell proliferation was 
      measured by means of tritiated thymidine incorporation. IgE was determined by 
      means of ELISA. Cell-cycle analysis was performed by using flow cytometry. 
      RESULTS: Both intact and fractionated IVIG inhibited anti-CD40- and 
      IL-4--stimulated IgE production in a dose-dependent manner. The maximal 
      inhibition was achieved at 67 micromol/L (eg, 10, 6, and 4 mg/mL for intact IVIG, 
      F[ab'](2), and Fc, respectively). The effect of F(ab')(2) was more pronounced 
      than that of Fc at equimolar concentrations. Similarly, both intact and 
      fragmented IVIG dose-dependently decreased tritiated thymidine incorporation. 
      F(ab')(2) was also more potent than Fc in this effect. Heat-aggregated IVIG 
      exhibited similar potency to regular IVIG in inhibiting B-cell proliferation. The 
      inhibitory effects of IVIG were unlikely to have been caused by the induction of 
      apoptosis because neither intact nor fractionated IVIG had a significant effect 
      on cell-cycle parameters at the concentrations used. CONCLUSION: These data 
      suggest that both F(ab')(2) and Fc portions contribute to the inhibition of in 
      vitro IgE production by IVIG. The role of the F(ab')(2) portion is more important 
      than that of the Fc portion.
FAU - Zhuang, Q
AU  - Zhuang Q
AD  - Division of Allergy and Immunology, Montreal Children's Hospital, 
      Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada.
FAU - Mazer, B
AU  - Mazer B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (CD40 Antigens)
RN  - 0 (Immunoglobulin Fab Fragments)
RN  - 0 (Immunoglobulin Fragments)
RN  - 0 (Immunoglobulins, Intravenous)
RN  - 0 (immunoglobulin Fv)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Antibodies, Anti-Idiotypic
MH  - B-Lymphocytes/cytology/*drug effects
MH  - CD40 Antigens/metabolism
MH  - Cell Division/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Immunoglobulin E/*biosynthesis
MH  - Immunoglobulin Fab Fragments/pharmacology
MH  - Immunoglobulin Fragments/*pharmacology
MH  - Immunoglobulins, Intravenous/*pharmacology
MH  - Interleukin-4/pharmacology
MH  - Models, Immunological
EDAT- 2001/08/10 10:00
MHDA- 2001/09/08 10:01
CRDT- 2001/08/10 10:00
PHST- 2001/08/10 10:00 [pubmed]
PHST- 2001/09/08 10:01 [medline]
PHST- 2001/08/10 10:00 [entrez]
AID - S0091-6749(01)90562-7 [pii]
AID - 10.1067/mai.2001.116291 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 2001 Aug;108(2):229-34. doi: 10.1067/mai.2001.116291.