PMID- 11496826
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20190901
IS  - 0015-5691 (Print)
IS  - 0015-5691 (Linking)
VI  - 118
IP  - 1
DP  - 2001 Jul
TI  - [Primary sensory neurons expressing histamine H1-receptor mRNA].
PG  - 43-9
AB  - Pharmacological studies have suggested that a subgroup of primary sensory neurons 
      is responsive to histamine via the H1 receptor. However, which type of primary 
      sensory neurons express H1 receptor is not known. We addressed this issue using 
      in situ hybridization histochemistry with a cRNA probe for the guinea pig H1 
      receptor mRNA. H1 receptor mRNA was expressed in about 15-20% of the trigeminal 
      and lumbar dorsal root ganglion (DRG) neurons, but none of the nodose ganglion 
      neurons. The positive neurons in DRG were exclusively small in size and were 
      labeled by isolectin B4, suggesting that these neurons have unmyelinated fibers. 
      However, H1-receptor mRNA-expressing DRG neurons were not immunoreactive to 
      substance P (SP) or calcitonin gene-related peptide (CGRP), which are implicated 
      in the nociceptive transmission of the primary sensory system. Moreover, in 
      guinea pigs neonatally treated with capsaicin (50 mg/kg), few CGRP-immunoreactive 
      neurons were seen in DRG, but the percentage of H1-receptor mRNA-expressing 
      neurons (15%-20%) and the intensity of the mRNA signals in these neurons were not 
      affected by neonatal capsaicin treatment, suggesting that H1 receptor-expressing 
      neurons are not sensitive to capsaicin. These findings suggest that 
      H1-receptor-expressing neurons are involved in the transmission of a unique 
      sensory modality such as itch. A marked increase in the number of mRNA-positive 
      DRG neurons was observed 1-5 days after a crush injury of the sciatic nerve 
      (3-4-fold of the control value). These neurons that turned mRNA-positive after 
      the nerve crush were also mainly small-sized. The mRNA signals were detected in 
      many peptidergic (SP/CGRP) neurons, in contrast to the normal condition. On the 
      other hand, mRNA signals were decreased in the neurons that showed intense 
      labeling in the normal condition. These results suggest that the gene expression 
      of H1 receptors up-regulated in injured afferents may be involved in neuropathic 
      pain.
FAU - Kashiba, H
AU  - Kashiba H
AD  - Department of Physiology, Kansai College of Oriental Medicine, 2-11-1 Wakaba, 
      Kumatori, Sennan, Osaka 590-0433, Japan.
FAU - Senba, E
AU  - Senba E
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Japan
TA  - Nihon Yakurigaku Zasshi
JT  - Nihon yakurigaku zasshi. Folia pharmacologica Japonica
JID - 0420550
RN  - 0 (Neuropeptides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Histamine H1)
RN  - 820484N8I3 (Histamine)
RN  - S07O44R1ZM (Capsaicin)
SB  - IM
MH  - Animals
MH  - Capsaicin/pharmacology
MH  - *Gene Expression
MH  - Histamine/metabolism
MH  - Humans
MH  - Neuralgia/genetics
MH  - *Neurons, Afferent/metabolism/physiology
MH  - Neuropeptides/physiology
MH  - RNA, Messenger/*metabolism
MH  - Receptors, Histamine H1/*genetics
MH  - Up-Regulation
RF  - 38
EDAT- 2001/08/11 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/08/11 10:00
PHST- 2001/08/11 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/08/11 10:00 [entrez]
AID - 10.1254/fpj.118.43 [doi]
PST - ppublish
SO  - Nihon Yakurigaku Zasshi. 2001 Jul;118(1):43-9. doi: 10.1254/fpj.118.43.