PMID- 11498245
OWN - NLM
STAT- MEDLINE
DCOM- 20010913
LR  - 20190901
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 81
IP  - 1-2
DP  - 2001 Aug 30
TI  - Changes in peripheral blood leukocyte populations in pigs with natural 
      postweaning multisystemic wasting syndrome (PMWS).
PG  - 37-44
AB  - The objective of the present study was to analyze, by flow cytometry, changes in 
      PBMC subsets in pigs having postweaning multisystemic wasting syndrome (PMWS), a 
      new condition associated to porcine circovirus type 2 (PCV2) infection. Thirteen 
      acutely PMWS affected pigs were selected from a farm seronegative to porcine 
      reproductive and respiratory syndrome virus (PRRSV) and to Aujeszky's disease 
      virus (ADV); 11 clinically healthy pigs were selected from a high health farm 
      with no history of PMWS and free of the major swine pathogens, and used as a 
      control group. All pigs were necropsied, and tissue samples were fixed in 
      formalin; blood with EDTA anticoagulant was used to perform the flow cytometric 
      analysis. PBMC were incubated with mAb against porcine CD3, CD4, CD8, CD25, CD45, 
      IgM, SWC3, and SLA-Class II. Flow cytometric analysis showed substantial changes 
      in leukocyte subsets in the peripheral blood of PMWS-affected pigs, which were 
      characterized by an increase of monocytes, a reduction of T (mainly CD4(+)) and 
      B-lymphocytes, and the presence of low-density immature granulocytes. Altogether, 
      these changes would suggest an inability of acutely PMWS-affected pigs to mount 
      an effective immune response.
FAU - Segales, J
AU  - Segales J
AD  - Departament de Sanitat i Anatomia Animals, Centre de Recerca en Sanitat Animal, 
      Universitat Autonoma de Barcelona, Bellaterra, 08193 Barcelona, Spain. 
      joaquim.segales@uab.es
FAU - Alonso, F
AU  - Alonso F
FAU - Rosell, C
AU  - Rosell C
FAU - Pastor, J
AU  - Pastor J
FAU - Chianini, F
AU  - Chianini F
FAU - Campos, E
AU  - Campos E
FAU - Lopez-Fuertes, L
AU  - Lopez-Fuertes L
FAU - Quintana, J
AU  - Quintana J
FAU - Rodriguez-Arrioja, G
AU  - Rodriguez-Arrioja G
FAU - Calsamiglia, M
AU  - Calsamiglia M
FAU - Pujols, J
AU  - Pujols J
FAU - Dominguez, J
AU  - Dominguez J
FAU - Domingo, M
AU  - Domingo M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Animals
MH  - Circoviridae Infections/immunology/*veterinary
MH  - Flow Cytometry
MH  - Immunoglobulin M/analysis
MH  - *Leukocyte Count
MH  - Swine
MH  - Swine Diseases/*immunology
MH  - Wasting Syndrome/immunology/*veterinary
MH  - Weaning
EDAT- 2001/08/11 10:00
MHDA- 2001/09/14 10:01
CRDT- 2001/08/11 10:00
PHST- 2001/08/11 10:00 [pubmed]
PHST- 2001/09/14 10:01 [medline]
PHST- 2001/08/11 10:00 [entrez]
AID - S0165242701003269 [pii]
AID - 10.1016/s0165-2427(01)00326-9 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2001 Aug 30;81(1-2):37-44. doi: 
      10.1016/s0165-2427(01)00326-9.