PMID- 11498805
OWN - NLM
STAT- MEDLINE
DCOM- 20010927
LR  - 20191025
IS  - 0895-8378 (Print)
IS  - 0895-8378 (Linking)
VI  - 13
IP  - 9
DP  - 2001 Sep
TI  - The influence of suspension nebulization or instillation on particle uptake by 
      guinea pig alveolar macrophages.
PG  - 773-88
AB  - Phagocytosis represents a crucial event in the host defense against pathogens. 
      Experimental methods are required that allow a range of particle doses to be 
      delivered. However, it is not clear that these methods result in the same sites 
      of deposition or mechanisms of clearance. The effect of particle administration 
      by nebulization or instillation on the uptake by guinea pig alveolar macrophages 
      (AMs) has been studied. Suspensions of escalating quantities of 1-microm 
      fluorescent polystyrene latex microspheres were delivered by 15 min of 
      nebulization (1.4 x 10(7)-11.1 x 10(7) particles) or instillation (19 x 10(7)-152 
      x 10(7) particles) into the lungs of guinea pigs. These doses were selected to 
      maximize delivery using each of these methods. Macrophages were collected by 
      alveolar lavage 6 h postadministration. The total number of cells recovered was 3 
      x 10(6) and the cell viability was >97%, which was measured by trypan blue 
      exclusion. Differential cell counts of lavaged cell suspensions were conducted 
      and results showed no difference for the two methods of administration with 
      various concentrations of latex particles and control samples. The uptake of 
      particles was measured using epifluorescence, confocal microscopy, and flow 
      cytometry. AMs showed a dose-dependent increase in associated particles measured 
      by microscopy and flow cytometry. There was a direct correlation (R(2) =.99) in 
      the phagocytic indices (PIs) measured by flow cytometry and fluorescence 
      microscopy. The PI was 15 times higher after instillation than that obtained 
      after particle nebulization. The percentage of AMs involved in phagocytosis 
      observed after instillation was 55% and after nebulization 23%. The uptake of 
      aerosolized particles by AMs and the number of cells involved in phagocytosis 
      were dependent on the particle dose and the efficiency of aerosol delivery to the 
      lungs.
FAU - Suarez, S
AU  - Suarez S
AD  - School of Pharmacy, University of North Carolina at Chapel Hill, Beard Hall CB 
      #7360, Chapel Hill, NC 27599, USA.
FAU - Kazantseva, M
AU  - Kazantseva M
FAU - Bhat, M
AU  - Bhat M
FAU - Costa, D
AU  - Costa D
FAU - Hickey, A J
AU  - Hickey AJ
LA  - eng
GR  - HL55789/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Inhal Toxicol
JT  - Inhalation toxicology
JID - 8910739
RN  - 0 (Latex)
RN  - 0 (Polystyrenes)
RN  - 0 (Xenobiotics)
SB  - IM
MH  - Administration, Inhalation
MH  - Animals
MH  - Cell Count
MH  - Cell Survival
MH  - Flow Cytometry
MH  - Guinea Pigs
MH  - Instillation, Drug
MH  - Latex
MH  - Macrophages, Alveolar/cytology/*metabolism
MH  - Microscopy, Fluorescence
MH  - Microspheres
MH  - Nebulizers and Vaporizers
MH  - Particle Size
MH  - Polystyrenes/administration & dosage/metabolism/toxicity
MH  - Xenobiotics/*administration & dosage/metabolism/toxicity
EDAT- 2001/08/11 10:00
MHDA- 2001/09/28 10:01
CRDT- 2001/08/11 10:00
PHST- 2001/08/11 10:00 [pubmed]
PHST- 2001/09/28 10:01 [medline]
PHST- 2001/08/11 10:00 [entrez]
AID - 10.1080/08958370120643 [doi]
PST - ppublish
SO  - Inhal Toxicol. 2001 Sep;13(9):773-88. doi: 10.1080/08958370120643.