PMID- 11500038
OWN - NLM
STAT- MEDLINE
DCOM- 20010920
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 286
IP  - 2
DP  - 2001 Aug 17
TI  - Direct observation of release of cytochrome c from lipid-encapsulated protein by 
      peroxide and superoxide: a possible mechanism for drug-induced apoptosis.
PG  - 311-4
AB  - Release of cytochrome c from inside lipid vesicles and from inside 
      proteoliposomes formed by cytochrome c oxidase has been studied by 
      spectrophotometric methods. The protein encapsulated inside vesicles did not form 
      complex with sodium azide solution added externally. Both hydrogen peroxide and 
      superoxide were found to cause release of cytochrome c from the lipid 
      encapsulated protein, which was detected from the distinct spectral changes due 
      to the formation of the azide complex of cytochrome c in the solution. Cytochrome 
      c encapsulated inside proteoliposomes containing cytochrome c oxidase (CcO) did 
      not release the cytochrome c during enzymatic turnover of CcO. The anticancer 
      drug, doxorubicin, was found to inhibit the biochemical function of cytochrome c 
      oxidase and release of cytochrome c was observed from the proteoliposome 
      encapsulating the protein during the enzymatic turnover in the presence of 
      doxorubicin. The results indicated that the inhibition of enzymatic activity by 
      doxorubicin possibly leads to the formation of reactive oxygen species, which 
      induce the release of cytochrome c from inside to outside of the membrane.
CI  - Copyright 2001 Academic Press.
FAU - Das, N
AU  - Das N
AD  - Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi 
      Bhabha Road, Mambai, 400005, India.
FAU - Gupta, S
AU  - Gupta S
FAU - Mazumdar, S
AU  - Mazumdar S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Cytochrome c Group)
RN  - 0 (Liposomes)
RN  - 0 (Proteolipids)
RN  - 0 (proteoliposomes)
RN  - 11062-77-4 (Superoxides)
RN  - 12030-88-5 (potassium superoxide)
RN  - 80168379AG (Doxorubicin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - *Apoptosis
MH  - Cytochrome c Group/*metabolism
MH  - Doxorubicin/pharmacology
MH  - Electron Transport Complex IV/metabolism
MH  - Hydrogen Peroxide/*pharmacology
MH  - Liposomes/metabolism
MH  - Proteolipids/metabolism
MH  - Superoxides/*pharmacology
EDAT- 2001/08/14 10:00
MHDA- 2001/09/21 10:01
CRDT- 2001/08/14 10:00
PHST- 2001/08/14 10:00 [pubmed]
PHST- 2001/09/21 10:01 [medline]
PHST- 2001/08/14 10:00 [entrez]
AID - S0006-291X(01)95403-8 [pii]
AID - 10.1006/bbrc.2001.5403 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2001 Aug 17;286(2):311-4. doi: 
      10.1006/bbrc.2001.5403.