PMID- 11500056
OWN - NLM
STAT- MEDLINE
DCOM- 20010920
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 286
IP  - 2
DP  - 2001 Aug 17
TI  - Menin interacts directly with the homeobox-containing protein Pem.
PG  - 426-31
AB  - The tumour suppressor gene causing multiple endocrine neoplasia type 1 (MEN1) 
      encodes a 610 amino acid protein, menin. In order to identify menin-interacting 
      proteins we used a yeast two-hybrid assay to screen a 12.5-dpc mouse embryo 
      library with partial menin encompassing amino acids 278 to 476. This identified a 
      homeobox containing protein encoded by a placenta and embryonic expression gene, 
      referred to as Pem. GST-pull-down and coimmunoprecipitation experiments confirmed 
      the interaction. Both proteins colocalised predominantly in the nucleus but were 
      occasionally also found in the cytoplasm. Furthermore, in situ hybridisation 
      studies revealed similarities in their expression patterns in mouse embryos and 
      adult tissues. In adult mice both Men1 and Pem yielded strong signals in testis, 
      Sertoli cells and particularly in seminiferous tubules. Thus, our study has 
      identified that menin interacts with Pem, and the high expression of these 
      proteins in the testis suggests a role in spermatogenesis.
CI  - Copyright 2001 Academic Press.
FAU - Lemmens, I H
AU  - Lemmens IH
AD  - Laboratory for Molecular Oncology and Flanders Interuniversity Institute for 
      Biotechnology, Center for Human Genetics, KU Leuven, Herestraat 49, Leuven, 
      B-3000, Belgium.
FAU - Forsberg, L
AU  - Forsberg L
FAU - Pannett, A A
AU  - Pannett AA
FAU - Meyen, E
AU  - Meyen E
FAU - Piehl, F
AU  - Piehl F
FAU - Turner, J J
AU  - Turner JJ
FAU - Van de Ven, W J
AU  - Van de Ven WJ
FAU - Thakker, R V
AU  - Thakker RV
FAU - Larsson, C
AU  - Larsson C
FAU - Kas, K
AU  - Kas K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Homeodomain Proteins)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Pem protein, mouse)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/metabolism
MH  - Fluorescent Antibody Technique
MH  - Homeodomain Proteins/genetics/*metabolism
MH  - In Situ Hybridization
MH  - Male
MH  - Mice
MH  - Neoplasm Proteins/genetics/*metabolism
MH  - *Proto-Oncogene Proteins
MH  - RNA, Messenger/biosynthesis
MH  - Spermatogenesis
MH  - Testis/metabolism
MH  - Transcription Factors/genetics/*metabolism
MH  - Two-Hybrid System Techniques
EDAT- 2001/08/14 10:00
MHDA- 2001/09/21 10:01
CRDT- 2001/08/14 10:00
PHST- 2001/08/14 10:00 [pubmed]
PHST- 2001/09/21 10:01 [medline]
PHST- 2001/08/14 10:00 [entrez]
AID - S0006-291X(01)95405-1 [pii]
AID - 10.1006/bbrc.2001.5405 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2001 Aug 17;286(2):426-31. doi: 
      10.1006/bbrc.2001.5405.